Large-Scale Preparation of Asn-Glycopeptide Carrying Structurally Homologous Antigenic N-Glycan
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In our previous report (M. Okano, <i>et al.</i>, <i>Clin. Exp. Allergy</i>, <b>34</b>, 770–778 (2004)), we found that free plant complex type <i>N</i>-glycans suppressed the production of IL4 from Th2 cells of Japanese cedar pollinosis patients, suggesting that plant complex type <i>N</i>-glycan can be used as a leading compound for developing immuno-pharmaceuticals. Although immunoreactive plant complex type <i>N</i>-glycans occur ubiquitously on glycoproteins expressed in plants, an appropriate procedure has not been established to prepare non-labeled immunoreactive glycans or glycopeptides bearing structurally homologous immunoreactive glycans in large amounts. In this study, therefore, we developed a new preparative procedure for the large-scale preparation of Asn-glycopeptide bearing plant complex type <i>N</i>-glycan using a combination of gel-filtration and the hydrophilic partitioning method. By this new method, about 103 mg of Asn-glycopeptide bearing the antigenic <i>N</i>-glycans was obtained from 1.9 kg of shelled <i>Ginkgo biloba</i> seeds.
- Bioscience, Biotechnology, and Biochemistry
Bioscience, Biotechnology, and Biochemistry 77(6), 1269-1274, 2013-06-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry