Large-Scale Preparation of Asn-Glycopeptide Carrying Structurally Homologous Antigenic N-Glycan

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Author(s)

    • MAEDA Megumi MAEDA Megumi
    • Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University
    • YAMANISHI Maomi
    • Faculty of Agriculture, Division of Agricultural Science, Okayama University
    • KIMURA Mariko
    • Faculty of Food Culture, Department of Nutrition, Kurashiki Sakuyo University
    • KIMURA Yoshinobu
    • Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University

Abstract

In our previous report (M. Okano, <i>et al.</i>, <i>Clin. Exp. Allergy</i>, <b>34</b>, 770–778 (2004)), we found that free plant complex type <i>N</i>-glycans suppressed the production of IL4 from Th2 cells of Japanese cedar pollinosis patients, suggesting that plant complex type <i>N</i>-glycan can be used as a leading compound for developing immuno-pharmaceuticals. Although immunoreactive plant complex type <i>N</i>-glycans occur ubiquitously on glycoproteins expressed in plants, an appropriate procedure has not been established to prepare non-labeled immunoreactive glycans or glycopeptides bearing structurally homologous immunoreactive glycans in large amounts. In this study, therefore, we developed a new preparative procedure for the large-scale preparation of Asn-glycopeptide bearing plant complex type <i>N</i>-glycan using a combination of gel-filtration and the hydrophilic partitioning method. By this new method, about 103 mg of Asn-glycopeptide bearing the antigenic <i>N</i>-glycans was obtained from 1.9 kg of shelled <i>Ginkgo biloba</i> seeds.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 77(6), 1269-1274, 2013-06-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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Codes

  • NII Article ID (NAID)
    10031184693
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168451
  • NDL Article ID
    024647125
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
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