Large-Scale Preparation of Asn-Glycopeptide Carrying Structurally Homologous Antigenic N-Glycan

• MAEDA Megumi

  Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University

• TAKEDA Naoto

  Faculty of Agriculture, Division of Agricultural Science, Okayama University

• MANO Aya

  Faculty of Agriculture, Division of Agricultural Science, Okayama University

• YAMANISHI Maomi

  Faculty of Agriculture, Division of Agricultural Science, Okayama University

• KIMURA Mariko

  Faculty of Food Culture, Department of Nutrition, Kurashiki Sakuyo University Faculty of Home Economics, Kobe Women's University

• KIMURA Yoshinobu

  Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University

書誌事項

タイトル別名

• Large-Scale Preparation of Asn-Glycopeptide Carrying Structurally Homologous Antigenic <i>N</i>-Glycan

抄録

In our previous report (M. Okano, et al., Clin. Exp. Allergy, 34, 770–778 (2004)), we found that free plant complex type N-glycans suppressed the production of IL4 from Th2 cells of Japanese cedar pollinosis patients, suggesting that plant complex type N-glycan can be used as a leading compound for developing immuno-pharmaceuticals. Although immunoreactive plant complex type N-glycans occur ubiquitously on glycoproteins expressed in plants, an appropriate procedure has not been established to prepare non-labeled immunoreactive glycans or glycopeptides bearing structurally homologous immunoreactive glycans in large amounts. In this study, therefore, we developed a new preparative procedure for the large-scale preparation of Asn-glycopeptide bearing plant complex type N-glycan using a combination of gel-filtration and the hydrophilic partitioning method. By this new method, about 103 mg of Asn-glycopeptide bearing the antigenic N-glycans was obtained from 1.9 kg of shelled Ginkgo biloba seeds.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (6), 1269-1274, 2013

  公益社団法人 日本農芸化学会

キーワード

• plant <i>N</i>-glycan
• immunoreactive glycan
• glycopeptide preparation
• neoglycoconjugate
• <i>Ginkgo biloba</i>

詳細情報

• CRID: 1390282681456499072
• NII論文ID: 10031184693
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.130076
• COI: 1:STN:280:DC%2BC3sjhtFWntg%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024647125
• PubMed: 23748790
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024647125
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.130076
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • PubMed
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  • KAKEN
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