Protective Effect of Luteolin on an Oxidative-Stress Model Induced by Microinjection of Sodium Nitroprusside in Mice

• Nazari Qand Agha

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan

• Kume Toshiaki

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan

• Takada-Takatori Yuki

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Doshisha Women's College, Japan

• Izumi Yasuhiko

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan

• Akaike Akinori

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan Graduate School of Pharmaceutical Sciences, Nagoya University, Japan

Abstract

Accumulating lines of evidence showed that luteolin, a polyphenolic compound, has potent neuroprotective effects. The purpose of this study was to examine whether luteolin can protect against sodium nitroprusside (SNP)-induced oxidative damage in mouse brain. Intrastriatal co-injection of luteolin (3 – 30 nmol) with SNP (10 nmol) dose-dependently protected against brain damage and motor dysfunction. Oral administrations of luteolin (600 – 1200 mg/kg) dose-dependently protected against brain damage and motor dysfunction induced by striatal injection of SNP. Furthermore, luteolin (30 – 100 μM) concentration dependently protected against Fe²⁺-induced lipid peroxidation in mouse brain homogenate. Luteolin (1 – 100 μg/ml) showed potent DPPH radical scavenging ability, when compared with ascorbic acid and glutathione. Finally, a ferrozine assay showed that luteolin (30 – 100 μg/ml) has Fe²⁺-chelating ability, but this was weaker than that of ethylenediaminetetraacetic acid. These results suggest that intrastriatal or oral administration of luteolin protected mice brain from SNP-induced oxidative damage by scavenging and chelating effects.

Journal

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 122 (2), 109-117, 2013

  The Japanese Pharmacological Society

Keywords

• sodium nitroprusside
• brain damage
• motor dysfunction
• oxidative stress
• scavenging

Details

• CRID: 1390001205180970368
• NII Article ID: 10031185403
• NII Book ID: AA11806667
• DOI: 10.1254/jphs.13019fp
• COI: 1:STN:280:DC%2BC3snmvVGisQ%3D%3D
• ISSN: 13478648
• ISSN: 13478613
• NDL BIB ID: 024637841
• PubMed: 23707972
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024637841
• Web Site: https://www.jstage.jst.go.jp/article/jphs/122/2/122_13019FP/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014145714
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed