Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) 2機種による血液培養分離菌の同定成績について  [in Japanese] Performance of Two Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Models for Identification of Bacteria Isolated from Blood Culture  [in Japanese]

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Author(s)

    • 伊藤 栄祐 ITOH Eisuke
    • 旭川医科大学病院臨床検査・輸血部 Department of Medical Laboratory and Blood Center, Asahikawa Medical University Hospital
    • 渡 智久 WATARI Tomohisa
    • 旭川医科大学病院臨床検査・輸血部 Department of Medical Laboratory and Blood Center, Asahikawa Medical University Hospital
    • 東 由桂 [他] AZUMA Yuka
    • 旭川医科大学病院臨床検査・輸血部 Department of Medical Laboratory and Blood Center, Asahikawa Medical University Hospital
    • 渡辺 直樹 WATANABE Naoki
    • 旭川医科大学病院臨床検査・輸血部 Department of Medical Laboratory and Blood Center, Asahikawa Medical University Hospital
    • 友田 豊 TOMODA Yutaka
    • 旭川医科大学病院臨床検査・輸血部 Department of Medical Laboratory and Blood Center, Asahikawa Medical University Hospital
    • 赤坂 和美 AKASAKA Kazumi
    • 旭川医科大学病院臨床検査・輸血部 Department of Medical Laboratory and Blood Center, Asahikawa Medical University Hospital
    • 紀野 修一 KINO Shuichi
    • 旭川医科大学病院臨床検査・輸血部 Department of Medical Laboratory and Blood Center, Asahikawa Medical University Hospital

Abstract

著者最終原稿版We compared the results of two bacterial identification methods: 1)a traditional method based on phenotypic identification of the causative organism using gram-staining, culture and biochemical markers and 2)matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 111 isolates, including 107 strains of common bacteria species and 4 strains of 3 yeast species, were tested by the traditional method and MALDI-TOF MS method (VITEK MS and Micro flex LT). Data obtained using MALDI-TOF MS were classified as Level 1 and Level 2 according to the confidence level of identification results from the VITEK MS ver. 1.0 database (VITEK MS) and MALDI Biotyper ver. 2.0 database (Microflex LT). The proportions of measured samples identified as Level 1 were 98.2% with the VITEK MS database and 87.4% with the MALDI Biotyper database. The concordance rates of the traditional method were 93.7% with the VITEK MS database and 82.0% with the MALDI Biotyper database. Identification results of five strains were mismatched between the traditional method and MALDI-TOF MS. Their ribosomal RNA sequences were identical to the results obtained from MALDI-TOF MS. We concluded that the performance of VITEK MS is superior to that of the traditional method and Microflex LT.

Journal

  • 臨床病理

    臨床病理 61(5), 382-389, 2013-05-25

    日本臨床検査医学会事務所 ; 1953-

References:  14

Codes

  • NII Article ID (NAID)
    10031185463
  • NII NACSIS-CAT ID (NCID)
    AN00253932
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    00471860
  • NDL Article ID
    024707655
  • NDL Call No.
    Z19-284
  • Data Source
    CJP  NDL  IR 
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