Adhesion and Internalization of Functionalized Polystyrene Latex Nanoparticles toward the Yeast <i>Saccharomyces cerevisiae</i>

• Miyazaki Jumpei

  Department of Chemical Engineering, Osaka Prefecture University

• Kuriyama Yuta

  Department of Chemical Engineering, Osaka Prefecture University

• Miyamoto Akihisa

  Department of Chemical Engineering, Osaka Prefecture University

• Tokumoto Hayato

• Konishi Yasuhiro

  Department of Chemical Engineering, Osaka Prefecture University

• Nomura Toshiyuki

  Department of Chemical Engineering, Osaka Prefecture University

Bibliographic Information

Other Title

• ポリスチレンラテックスナノ粒子の酵母細胞への付着・取込現象の評価
• ポリスチレンラテックスナノ リュウシ ノ コウボ サイボウ エ ノ フチャク ・ トリコミ ゲンショウ ノ ヒョウカ
• Adhesion and Internalization of Functionalized Polystyrene Latex Nanoparticles toward the Yeast Saccharomyces cerevisiae

Abstract

The toxicity and behavior (internalization, adhesion, dispersion) of manufactured polystyrene latex (PSL) nanoparticles (nominal diameter : 50 nm) with various functional groups toward the yeast Saccharomyces cerevisiae as a model eukaryote were examined using the colony count method and microscopic observation. The colony count tests suggested that the PSL nanoparticles with a negative surface charge had little or no toxicity toward the yeast. In contrast, PSL nanoparticles with an amine functional group and a positive surface charge (p-Amine) displayed a high toxicity in 5 mol/m³ NaCl, whereas the p-Amine were mostly unharmed in 154 mol/m³ NaCl, which was quite different with the toxicological effects of Escherichia coli as a model prokaryote. Confocal and atomic force microscopy indicated that in 5 mol/m³ NaCl the p-Amine nanoparticles entirely covered the surface of the yeast, and cell death occurred, whereas in 154 mol/m³ NaCl were internalized by endocytosis, and cell death did not occur.

Journal

• Journal of the Society of Powder Technology, Japan

  Journal of the Society of Powder Technology, Japan 50 (7), 472-477, 2013

  The Society of Powder Technology, Japan

Keywords

• Toxicity
• Polystyrene latex nanoparticle
• Internalization
• Adhesion
• Surface potential
• <i>Saccha romyces cerevisiae</i>

Details

• CRID: 1390001204511773568
• NII Article ID: 10031185746
• NII Book ID: AN00222757
• DOI: 10.4164/sptj.50.472
• COI: 1:CAS:528:DC%2BC3sXhtlWrtLvI
• ISSN: 18837239
• ISSN: 03866157
• NDL BIB ID: 024761654
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024761654
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed