Quantification of Candesartan in Mouse Plasma by MALDI-TOFMS and in Tissue Sections by MALDI-Imaging Using the Stable-Isotope Dilution Technique
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- Nakanishi Toyofumi
- Department of Clinical and Laboratory Medicine, Osaka Medical College
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- Takai Shinji
- Department of Pharmacology, Osaka Medical College
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- Jin Denan
- Department of Pharmacology, Osaka Medical College
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- Takubo Takayuki
- Department of Clinical and Laboratory Medicine, Osaka Medical College
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Abstract
To determine the contents of candesartan in mouse plasma, and blood vessel and kidney sliced sections and also better understand its pharmacokinetics, we applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and MALDI-imaging mass spectrometry (IMS) with the selected reaction monitoring (SRM) mode using a labeled-internal standard. The results of fundamental examinations showed that the slope of the resulting curves of candesartan in the plasma from the equation was 0.91 and the y-intercept was 0.02. Both intra- and inter-day accuracies (n=10) and the precision of candesartan in the plasma by MALDI-TOFMS with the SRM mode were in the range of 3.4 to 17.3% and 93.2%, respectively. The detection limit of candesartan in spiked plasma was 0.2 nmol/L. IMS analysis enabled us to clarify distinct spacial time-distribution images in sliced mouse blood vessel and kidney sections although it still needed to improve a protocol of quantification. Typical pharmacokinetic patterns of candesartan were obtained in the plasma and sliced kidney sections, but those in the blood vessel sections gradually increased 24 h after administration. MALDI-TOFMS and IMS with the SRM mode are powerful tools to identify the spacial distribution and traceability of candesartan in sliced blood vessel and tissue sections as well as in the plasma.
Journal
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- Mass Spectrometry
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Mass Spectrometry 2 (1), A0021-A0021, 2013
The Mass Spectrometry Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282680392872960
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- NII Article ID
- 10031189344
- 130003380163
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- NII Book ID
- AA12588153
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- ISSN
- 21865116
- 2187137X
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed