The Fission Yeast <i>php2</i> Mutant Displays a Lengthened Chronological Lifespan
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- TAKUMA Kazuaki
- Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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- OHTSUKA Hokuto
- Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University
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- AZUMA Kenko
- Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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- MURAKAMI Hiroshi
- Department of Biological Science, Faculty of Science and Engineering, Chuo University
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- AIBA Hirofumi
- Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University
Bibliographic Information
- Other Title
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- The Fission Yeast php2 Mutant Displays a Lengthened Chronological Lifespan
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Abstract
The Schizosaccharomyces pombe php2+ gene encodes a subunit of the CCAAT-binding factor complex. We found that disruption of the php2+ gene extended the chronological lifespan of the fission yeast. Moreover, the lifespan of the Δphp2 mutant was barely extended under calorie restricted (CR) conditions. Many other phenotypes of the Δphp2 mutant resembled those of wild-type cells grown under CR conditions, suggesting that the Δphp2 mutant might undergo CR. The mutant also showed low respiratory activity concomitant with decreased expression of the cyc1+ and rip1+ genes, both of which are involved in mitochondrial electron transport. On the basis of a chromatin immunoprecipitation assay, we determined that Php2 binds to a DNA region upstream of cyc1+ and rip1+ in S. pombe. Here we discuss the possible mechanisms by which the chronological lifespan of Δphp2 mutant is extended.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (7), 1548-1555, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details
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- CRID
- 1390282681455718912
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- NII Article ID
- 10031190467
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- NII Book ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3sjos1eisQ%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 024723759
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- PubMed
- 23832353
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed