The Fission Yeast php2 Mutant displays a Lengthened Chronological Lifespan

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Author(s)

    • OHTSUKA Hokuto OHTSUKA Hokuto
    • Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University
    • MURAKAMI Hiroshi
    • Department of Biological Science, Faculty of Science and Engineering, Chuo University
    • AIBA Hirofumi
    • Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University

Abstract

The <i>Schizosaccharomyces pombe</i> <i>php2</i><sup>+</sup> gene encodes a subunit of the CCAAT-binding factor complex. We found that disruption of the <i>php2</i><sup>+</sup> gene extended the chronological lifespan of the fission yeast. Moreover, the lifespan of the Δ<i>php2</i> mutant was barely extended under calorie restricted (CR) conditions. Many other phenotypes of the Δ<i>php2</i> mutant resembled those of wild-type cells grown under CR conditions, suggesting that the Δ<i>php2</i> mutant might undergo CR. The mutant also showed low respiratory activity concomitant with decreased expression of the <i>cyc1</i><sup>+</sup> and <i>rip1</i><sup>+</sup> genes, both of which are involved in mitochondrial electron transport. On the basis of a chromatin immunoprecipitation assay, we determined that Php2 binds to a DNA region upstream of <i>cyc1</i><sup>+</sup> and <i>rip1</i><sup>+</sup> in <i>S. pombe</i>. Here we discuss the possible mechanisms by which the chronological lifespan of Δ<i>php2</i> mutant is extended.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 77(7), 1548-1555, 2013-07-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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Codes

  • NII Article ID (NAID)
    10031190467
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168451
  • NDL Article ID
    024723759
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
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