The Fission Yeast <i>php2</i> Mutant Displays a Lengthened Chronological Lifespan

  • TAKUMA Kazuaki
    Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
  • OHTSUKA Hokuto
    Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University
  • AZUMA Kenko
    Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
  • MURAKAMI Hiroshi
    Department of Biological Science, Faculty of Science and Engineering, Chuo University
  • AIBA Hirofumi
    Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University

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  • The Fission Yeast php2 Mutant Displays a Lengthened Chronological Lifespan

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Abstract

The Schizosaccharomyces pombe php2+ gene encodes a subunit of the CCAAT-binding factor complex. We found that disruption of the php2+ gene extended the chronological lifespan of the fission yeast. Moreover, the lifespan of the Δphp2 mutant was barely extended under calorie restricted (CR) conditions. Many other phenotypes of the Δphp2 mutant resembled those of wild-type cells grown under CR conditions, suggesting that the Δphp2 mutant might undergo CR. The mutant also showed low respiratory activity concomitant with decreased expression of the cyc1+ and rip1+ genes, both of which are involved in mitochondrial electron transport. On the basis of a chromatin immunoprecipitation assay, we determined that Php2 binds to a DNA region upstream of cyc1+ and rip1+ in S. pombe. Here we discuss the possible mechanisms by which the chronological lifespan of Δphp2 mutant is extended.

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