Chromatographic Behavior of Selenoproteins in Rat Serum Detected by Inductively Coupled Plasma Mass Spectrometry

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Abstract

Two major selenoproteins are present in mammalian serum: extracellular glutathione peroxidase (eGPx) and selenoprotein P (Sel P). The chromatographic behaviors of the two serum selenoproteins were compared in four rodent species, and the selenoproteins in rat serum were identified by measuring enzyme activity and Western blotting. The selenoproteins in rat serum showed a specific chromatographic behavior. In particular, rat eGPx was eluted faster than eGPxs of the other rodent species, although the amino-acid sequences of the rodent species were identical. The elution profiles of Se in rat serum obtained by inductively coupled plasma tandem mass spectrometry (ICP-MS-MS) and ICP-MS were compared. The tandem quadrupoles and the O<sub>2</sub> reaction/collision gas completely removed severe interferences with the Se speciation originating from the plasma source and the biological sample matrix. ICP-MS-MS under the O<sub>2</sub> mass shift mode gave us more accurate abundance ratios of Se than ICP-MS.

Journal

  • Analytical Sciences

    Analytical Sciences 29(8), 787-792, 2013-08-10

    The Japan Society for Analytical Chemistry

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Codes

  • NII Article ID (NAID)
    10031190632
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09106340
  • NDL Article ID
    024737586
  • NDL Call No.
    Z54-F482
  • Data Source
    CJP  NDL  J-STAGE 
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