Enhancing Effect of a Cysteinyl Thiol on the Antioxidant Activity of Flavonoids and Identification of the Antioxidative Thiol Adducts of Myricetin

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  • MASUDA Toshiya
    Graduate School of Integrated Arts and Sciences, University of Tokushima
  • MIURA Yukari
    Graduate School of Integrated Arts and Sciences, University of Tokushima
  • INAI Miyuki
    Graduate School of Integrated Arts and Sciences, University of Tokushima
  • MASUDA Akiko
    Faculty of Human Life Sciences, Shikoku University

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The enhancing effect of a cysteinyl thiol N-benzoylcysteine methyl ester on the antioxidant activity of several flavonoids was investigated in a lipid oxidation system. Obvious enhancement was apparent for catechin, myricetin, quercetin, and taxifolin, the activity for myricetin being the most potent among them. An HPLC analysis of the products from the antioxidation reaction of myricetin in the presence of the thiol was carried out and the structures of the products were determined to clarify the enhancing effect chemically. The obtained data indicated that two thiol adducts on the B ring, and probably C-ring adducts, which were produced in the antioxidation process, exerted an enhancing effect on the antioxidant activity of myricetin.

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