Molecular Cloning and Bacterial Expression of the Catalytic Domain of the SENP1 Gene of <i>Oryzias latipes</i>

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  • OBATA Shinpei
    Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University
  • YUASA Eri
    Faculty of Sciences, Kumamoto University
  • SEKI Daisuke
    Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University
  • KITANO Takeshi
    Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University
  • SAITOH Hisato
    Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University Faculty of Sciences, Kumamoto University Department of New Frontier Sciences, Graduate School of Science and Technology, Kumamoto University

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  • Molecular Cloning and Bacterial Expression of the Catalytic Domain of the SENP1 Gene of Oryzias latipes

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Abstract

In this study, we cloned the catalytic domain of the Oryzias latipes sentrin/SUMO-specific protease 1 (OlSENP1-CD) gene and produced the recombinant OlSENP1-CD protein in Escherichia coli. Experimental procedures designed to reveal the ability of the recombinant protein to show deSUMOylating activity in vitro should be helpful in future studies of other SENPs and the SUMO pathway.

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