Dynamics of Living Cells Measured by Scanning Probe Microscopy

• MIZUTANI Takeomi

  Faculty of Advanced Life Sciences, Hokkaido University

• KAWABATA Kazushige

  Faculty of Advanced Life Sciences, Hokkaido University

Bibliographic Information

Other Title

• 細胞のダイナミクス
• サイボウ ノ ダイナミクス

Abstract

Atomic force microscope (AFM) has advantages in the measurement of mechanical properties of soft materials. Here, we introduced applications of AFM to elucidate a single cell mechanics and multi-cellular dynamics. Comparison of elasticity map of fibroblasts with distribution of intracellular cytoskeletal fibers showed correspondence of high elasticity regions andactin fiber distributions. A representative morphology of cellular membrane ruffling was clearly observed and elasticity of the ruffling regions was higher than that of the outside of the ruffling regions. Elasticity map of epithelial cells showed cell-cell contact point is high elasticity. Cellular elasticity measurements by AFM will be applied to medical usages and some modifications of AFM will open a new door to study dynamics of soft materials.

Journal

• Hyomen Kagaku

  Hyomen Kagaku 34 (9), 500-504, 2013

  The Surface Science Society of Japan

Keywords

• elasticity
• cell
• cytoskeleton
• membrane ruffling

Details

• CRID: 1390001206459219968
• NII Article ID: 10031195952
• NII Book ID: AN00334149
• DOI: 10.1380/jsssj.34.500
• ISSN: 18814743
• ISSN: 03885321
• NDL BIB ID: 024879194
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024879194
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed