Rational Design of a Fluorescence-amplified Aptasensor Based on Enzyme-assisted Target Recycling Strategy

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  • Shingo Nakao
    Division of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuba
  • Motoi Oishi
    Division of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuba

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<jats:title>Abstract</jats:title> <jats:p>A fluorescence-amplified sensing system composed of a fluorophore-labeled adenosine DNA-aptamer/quencher-labeled capture DNA duplex, assistant DNA, and exonuclease III (Exo III) was designed and prepared. The newly constructed enzyme-assisted aptasensor exhibited amplification of fluorescent signal in response to adenosine (target) through an Exo III-assisted target recycling process.</jats:p>

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  • Chemistry Letters

    Chemistry Letters 42 (10), 1122-1124, 2013-06-08

    Oxford University Press (OUP)

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