Isolation and characterisation of the ADP-glucose pyrophosphorylase small subunit gene (AgpS1) promoter in tomato (Solanum lycopersicum L.)

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Abstract

ADP-glucose pyrophosphorylase (AGPase) is a key regulatory enzyme in starch biosynthesis. In this research, 2,885 bp of the predicted promoter sequence for the <i>AgpS1</i> gene encoding the AGPase small subunit was isolated from tomato. Sequence analyses revealed a number of known <i>cis</i>-elements related to responses to salt and dehydration stress and sugar repression; predicted TATA boxes are located at −88 to −94 bp and −114 to −120 bp. The spatial expression pattern and tissue/organ specificity of <i>AgpS1</i> were analysed in during development using promoter-GUS transgenic tomato plants. Based on GUS staining, the obtained sequence was proven to be the functional promoter and directed broad expression in both sink and source tissues/organs, including seedling, stem, flower, fruit stalk, fruit and root. In source leaf and early developing fruit, GUS staining was observed in all tissues, except for epidermal tissue. In contrast, GUS staining tended to be confined to vascular tissues in seedling, stem, fruit stalk and ripening fruit. In particular, a patchy staining pattern was observed in the phloem of the stem and fruit stalk, suggesting that <i>AgpS1</i> is expressed in the phloem companion cells in those organs. These results also suggest that AGPase mainly functions in the vascular tissue of those organs.

Journal

  • Plant Biotechnology

    Plant Biotechnology 30(3), 279-286, 2013-09-25

    Japanese Society for Plant Cell and Molecular Biology

References:  26

Codes

  • NII Article ID (NAID)
    10031202046
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13424580
  • NDL Article ID
    024902708
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  NDL  J-STAGE 
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