CAD2 deficiency causes both brown midrib and gold hull and internode phenotypes in Oryza sativa L. cv. Nipponbare

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Abstract

Several <i>brown midrib</i> (<i>bm</i>) mutants have so far been isolated from the C4 grasses, maize, sorghum and pearl millet, but have not been detected in C3 grasses including rice (<i>Oryza sativa</i>). In the present study we characterized the <i>cad2</i> (<i>cinnamyl alcohol dehydrogenase 2</i>) null mutant isolated from retrotransposon <i>Tos17</i> insertion lines of <i>Oryza sativa</i> L. ssp. <i>japonica</i> cv. Nipponbare. This mutant exhibited brown-colored midribs in addition to hulls and internodes, clearly indicating both <i>bm</i> and <i>gold hull and internode</i> (<i>gh</i>) phenotypes. The enzymatic saccharification efficiency in the culm of <i>cad2</i> null mutant was increased by 16.1% than that of the control plants. The lignin content of the <i>cad2</i> null mutant was 14.6% lower than that of the control plants. Thioacidolysis of the <i>cad2</i> null mutant indicated the presence of cinnamaldehyde structures in the lignin. Taken together, our results show that deficiency of <i>OsCAD2</i> causes the <i>bm</i> phenotype in addition to <i>gh</i>, and that the coloration is probably due to the accumulation of cinnamaldehyde-related structures in the lignin. Additionally, this <i>cad2</i> null mutant was useful to silage purposes and biofuel production.

Journal

  • Plant Biotechnology

    Plant Biotechnology 30(4), 365-373, 2013-09-25

    Japanese Society for Plant Cell and Molecular Biology

References:  53

Codes

  • NII Article ID (NAID)
    10031202056
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13424580
  • NDL Article ID
    024901815
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  NDL  J-STAGE 
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