ASYMMETRIC LEAVES2 and FASCIATA2 cooperatively regulate the formation of leaf adaxial-abaxial polarity in Arabidopsis thaliana

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Abstract

The establishment of adaxial–abaxial polarity in the early stage of leaf development is important for the expansion of lamina. In <i>Arabidopsis thaliana</i>, <i>asymmetric leaves2</i> (<i>as2</i>) and <i>as1</i> mutations cause defects in the leaf adaxial–abaxial polarity, which are exhibited as abaxialized filamentous leaves in the various modifier mutant backgrounds. Several modifier single mutants generate pointed leaves in common. Mutations in <i>FASCIATA2</i> (<i>FAS2</i>) also cause pointed leaves. The <i>FAS2</i> gene encodes a component of Chromatin Assembly Factor-1 (CAF-1), a histone chaperone, which affects mRNA levels of various genes through the regulation of chromatin states. In the present study, we demonstrate that <i>as2 fas2</i> double mutants frequently generate abaxialized filamentous leaves and show increased mRNA levels of genes for leaf abaxialization, <i>KANADI1</i> (<i>KAN1</i>), <i>KAN2</i>, <i>YABBY5</i> (<i>YAB5</i>), <i>ETTIN/AUXIN RESPONSE FACTOR3</i> (<i>ETT</i>/<i>ARF3</i>) and <i>ARF4</i>. In addition, the transcript levels of all four class 1 <i>KNOTTED1-like</i> homeobox genes that are involved in the maintenance of shoot apical meristem and the <i>Kip-related Protein2</i> (<i>KRP2</i>) and <i>KRP5</i> genes that are involved in the cell cycle progression are elevated in the <i>as2 fas2</i>. The mRNA levels of all genes other than <i>YAB5</i>, whose transcript levels rise in <i>as2 fas2</i>, were increased in the <i>fas2</i> single mutants. Our data suggest that FAS2 participates in the establishment of leaf adaxial–abaxial polarity through the repression of the transcript levels of these genes on the <i>as2</i> background.

Journal

  • Plant Biotechnology

    Plant Biotechnology 30(4), 411-415, 2013-09-25

    Japanese Society for Plant Cell and Molecular Biology

References:  43

Codes

  • NII Article ID (NAID)
    10031202063
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    NOT
  • ISSN
    13424580
  • NDL Article ID
    024901946
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  NDL  J-STAGE 
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