INHIBITION OF MYOGENIC DIFFERENTIATION BY DELTA-NOTCH SIGNALING PATHWAY

  • 松林 正
    Laboratory of Immunology, Aichi Cancer Center Research Institute Department of Pediatrics, Nagoya City University Medical School
  • 水谷 圭吾
    Laboratory of Immunology, Aichi Cancer Center Research Institute Department of Pediatrics, Nagoya City University Medical School
  • 矢崎 信
    Department of Pediatrics, Nagoya City University Medical School
  • 和田 義郎
    Department of Pediatrics, Nagoya City University Medical School
  • 高橋 利忠
    Laboratory of Immunology, Aichi Cancer Center Research Institute
  • 小幡 裕一
    Laboratory of Immunology, Aichi Cancer Center Research Institute

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抄録

The Delta/Serrate-Notch pathway mediates the signals of cell to cell communication during development in order to determine the fate of cells with equal potentials. However, the roles of Delta in mammalian development have not been fully understood. To delineate the functions of the mammalian Delta, we isolated a full-length cDNA of a mouse Delta homologue, mDelta 1,and transfected it into L cells using a eukaryotic expression vector. After confirming the expression of mDelta 1 in transfected L cells by Northern blot analysis, we test whether Delta had any effects on the differentiation of mammalian cells by coculturing C2C12 myogenic cells, which are known to differentiate into myocytes after serum starvation, on the mDelta 1 transfected L cells. When cultured on sense mDelta 1 transfectants, C2C12 remained undifferentiated even after the induction of differentiation. On the other hand, those on antisense mDelta 1 transfectants displayed characteristic morphological changes of myocyte differentiation. These results showed that mDelta 1 has an activity of regulating cellular differentiation.

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