LIVER REGENERATION FOLLOWING PARTIAL HEPATECTOMY IN THE SUBTOTAL PANCREATIC DUCT LIGATION RAT MODEL

  • 趙 来源
    The First Department of Surgery, Nagoya City University Medical School
  • 橋本 俊
    The First Department of Surgery, Nagoya City University Medical School
  • 鈴木 達也
    The First Department of Surgery, Nagoya City University Medical School
  • 真辺 忠夫
    The First Department of Surgery, Nagoya City University Medical School

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To further investigate roles of the exocrine pancreas in liver regeneration, we conducted a study with a subtotal pancreatic duct ligation model in rats. [Materials and methods] Eighty SD rats were divided into four groups receiving the following treatments : control group (sham laparotomy); subtotal pancreatic duct ligation (PL); 68% heptectomy (Hx); and PL plus Hx (PL+Hx). The surgical schedule was divided into two stages : stage one, subtotal ligation of pancreatic duct was performed for Groups 2 (PL) and 4 (PL+Hx); stage two, 68% hepatectomy was performed for Groups 3 (Hx) and 4 (PL+Hx) one week later. On postoperation days 1,2,3 and 7,five rats of each group were sacrificed. Liver wet weight (LWW) was measured and liver regeneration rate (LRR) was calculated. BrdU-labeling and mitotic indices in liver were examined, and insulin and HGF concentrations in serum were also measured. [Results] Compared with the Hx group, the LRR in the PL+Hx group markedly decreased on postoperation days 1 and 3. The peak of hepatocyte DNA synthesis was also suppressed with mitosis showing significant reduction and delay, the insulin concentration on postoperation day 1 was decreased and the peak of HGF showed delay and depression.[Conclusions] Subtotal pancreatic duct ligation suppresses liver regeneration in the initial period. This is related to changes in HGF and insulin serum concentrations and suggests that the exocrine pancreas provides factor supporting early liver regeneration.

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