A deoxyribonuclease I from rice bran

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A method of purification of a deoxyribonuclease I from rice bran was devised. Though it is not the final, the enzyme preparation is purified about 16,000 fold with respect to deoxyribonuclease activity, and the contamination of phosphatase is reduced to a trace. The pH optimum is 5.5. No metal ion is required. Deoxyguanylic acid alone was missing among 5’-mononucleotides identified in the digestion products. The enzyme action appears endonucleolytic.

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詳細情報 詳細情報について

  • CRID
    1390853649614585088
  • NII論文ID
    110000017181
  • NII書誌ID
    AA00247166
  • DOI
    10.5109/22727
  • HANDLE
    2324/22727
  • ISSN
    00236152
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • IRDB
    • Crossref
    • CiNii Articles

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