Silkworm Nuclease-Its Nature and Promise

• 向井 純一郎

  九州大学農学部農芸化学科蚕糸化学講座

抄録

An endonuclease was purified from the digestive juice of silkworm larvae, Bombyx mori. The enzyme hydrolyses both DNA and RNA endolytically with little base specificity, yielding di- and trinucleotides terminating in 5'-phosphate. Polynucleotides terminated in 3' -phosphate gave dinucleosidemonophosphates and trinucleosidediphosphates from 5'-end, di- and trinucleotides from inside, and mononucleosidediphosphates, dinucleosidetriphosphates, and trinucleosidetetraphosphates from 3'-end, respectively. Taking advantage of these peculiar nature of the nuclease, an oligonucleotide addition procedure was devised to sequence nucleic acids from the overlapping pattern of the fragments of digestion by the nuclease. Proven and potential usefulness of the silkworm endonuclease in the structural work on nucleic acids and specificity analysis of nucleolytic enzymes were discussed.

収録刊行物

• Journal of the Faculty of Agriculture, Kyushu University

  Journal of the Faculty of Agriculture, Kyushu University 17 (1), 21-29, 1972-08

  九州大学大学院農学研究院

キーワード

• Silkworm
• Nuclease
• Enzyme
• Nature
• Promise

詳細情報

• CRID: 1390572174637853184
• NII論文ID: 110000017269
• NII書誌ID: AA00247166
• DOI: 10.5109/22815
• HANDLE: 2324/22815
• ISSN: 00236152
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles