Questionable Plasmid Involvement in Streptomyces ATP Nucleotide 3’-Pyrophosphokinase Formation

• 牟田 滋

  九州大学農学部大学院農学研究科遺伝子資源工学専攻遺伝子制御学講座

• Mizui Yoshiharu

  Department of Agricultural Chemistry, Kyushu University

• Sumichika Hiroshi

  Department of Agricultural Chemistry, Kyushu University

• 岸原 健二

  九州大学生体防御医学研究所

• 向井 純一郎

  九州大学農学部大学院農学研究科遺伝子資源工学専攻遺伝子制御学講座

抄録

A few actinomycetes produce ATP nucleotide 3’-pyrophosphokinase, a mechanistic stringent factor analog with broad acceptor spectrum. The plasmid-like DNA of an ATP nucleotide S’mpyrophosphokinase producer Streptomyces violascens was more persistent than the enzyme production after acriflavin treatment. A 7.lKbp long ATP nucleotide 3’- pyrophosphokinase gene-containing fragment, cloned from another producer Stwptomyces more-okaensis, was found to hybridize to both its Xba I- and Spe I-digested genomic DNA at about 400Kbp size, and also with the undigested DNA very close to or at the origin on pulsed field gel electrophoresis-Southern blotting analyses. The gene thus appears to reside not on a plasmid, but on the chromosome.

収録刊行物

• Journal of the Faculty of Agriculture, Kyushu University

  Journal of the Faculty of Agriculture, Kyushu University 36 (1/2), 29-35, 1991-10

  Faculty of Agriculture, Kyushu University

キーワード

• ATP nucleotide 3'-pyrophosphokinase
• Streptomyces
• Plasmid

詳細情報

• CRID: 1390290699660971520
• NII論文ID: 110000017691
• NII書誌ID: AA00247166
• DOI: 10.5109/23965
• HANDLE: 2324/23965
• ISSN: 00236152
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles