Homologous Recombination of Mouse ZAKI-4 Gene to Disrupt its Expression

• KANOU Yasuhiko

• ABE Naoki

• ISHIDA Junji

• FUKAMIZU Akiyoshi

• SEO Hisao

• MURATA Yoshiharu

抄録

ZAKI-4 inhibits the activity of calcineurin, a Ca^<2+>-dependent protein phosphatase. From ZAKI-4 gene, two isoforms, a and P are generated by an alternative splicing. In adult mice ZAKI-4 α mRNA was mainly expressed in brain whereas ZAKI-4 β mRNA wasubiquitously. To elucidate the specific function of ZAKI-4 isoforms, we plan to establish ZAKI-4 β knock out mice by homologousrecombination. For this purpose, mouse embryonic stem cells were electroporated with a targeting vector in which ZAKI-4 β sequencewas disrupted by cDNA coding neomycin resistance. Six independent clones out of 466 antibiotics-resistant colonies underwenthomologous recombination at the ZAKI-4 β locus. These clones will be used to establish the knock out mice.

収録刊行物

• Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University

  Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University 46 (1/2), 55-57, 2002-12

  Research Institute of Environmental Medicine, Nagoya University

キーワード

• ZAKI-4
• homologous recombination
• gene targeting
• calcineurin

詳細情報

• CRID: 1390572174357584896
• NII論文ID: 110000029083
• NII書誌ID: AA1051621X
• DOI: 10.18999/envm.46.55
• HANDLE: 2237/2783
• ISSN: 02870517
• Web Site: https://nagoya.repo.nii.ac.jp/records/1373
• Web Site: https://search.jamas.or.jp/link/ui/2003221333
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • CiNii Articles
• 抄録ライセンスフラグ: 使用可