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Abstract
ZAKI-4 inhibits the activity of calcineurin, a Ca^<2+>-dependent protein phosphatase. From ZAKI-4 gene, two isoforms, a and P are generated by an alternative splicing. In adult mice ZAKI-4 α mRNA was mainly expressed in brain whereas ZAKI-4 β mRNA wasubiquitously. To elucidate the specific function of ZAKI-4 isoforms, we plan to establish ZAKI-4 β knock out mice by homologousrecombination. For this purpose, mouse embryonic stem cells were electroporated with a targeting vector in which ZAKI-4 β sequencewas disrupted by cDNA coding neomycin resistance. Six independent clones out of 466 antibiotics-resistant colonies underwenthomologous recombination at the ZAKI-4 β locus. These clones will be used to establish the knock out mice.
Journal
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- Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University
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Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University 46 (1/2), 55-57, 2002-12
Research Institute of Environmental Medicine, Nagoya University
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Details 詳細情報について
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- CRID
- 1390572174357584896
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- NII Article ID
- 110000029083
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- NII Book ID
- AA1051621X
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- HANDLE
- 2237/2783
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- ISSN
- 02870517
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- CiNii Articles
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- Abstract License Flag
- Allowed