胚珠培養,胚培養による3倍体マスカット個体の育成〔英文〕

• Okamoto, Goro

• Hirano, Ken

• Tanakamaru, Naomi

• Omori, Naoki

書誌事項

タイトル別名

• Obtaining Triploid Muscat Grapes by in vitro Culture of Ovules and Embryos After Crossing Between Diploid and Tetraploid Cultivars
• ハイシュ バイヨウ ハイ バイヨウ ニ ヨル 3バイタイ マスカット コタイ
• 胚珠培養、胚培養による3倍体マスカット個体の育成

抄録

To breed new seedless grape cultivars we crossed 'Muscat of Alexandria' (2x) and its 4x mutant reciprocally and cultured the immature ovules and embryos in vitro. Ovules sampled 50 days after crossing could develop embryos on a liquid medium of half strength of MS supplemented with 2 ppm of IAA, 0.4 ppm of GA3 and 0.1 % of activated charcoal. After 55 days culture, normally developed embryos were obtained from 20 to 30 % of the ovules from 4x×2x and 50 to 70 % of those from 2x×4x. Embryos taken out from the cultured ovules were planted on an agar medium of MS or N&N for 55 to 65 days. Ten to twenty % of embryos from 2x×4x crossing grew to rooted plantlets successfully. They were proved to be triploid after the measurement of chromosome number. Embryos from 4x×x2x, however, could not grow to plantlets because of the failure of rooting.

収録刊行物

• 岡山大学農学部学術報告

  岡山大学農学部学術報告 82 (1), 25-29, 1993

  岡山大学農学部

キーワード

• 胚珠培養
• 胚培養
• マスカット個体
• 育成

詳細情報

• CRID: 1050846637352827520
• NII論文ID: 110000130348
• NII書誌ID: AN00033029
• ISSN: 04740254
• NDL書誌ID: 3603382
• Web Site: https://ousar.lib.okayama-u.ac.jp/910
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3603382
• 本文言語コード: en
• 資料種別: departmental bulletin paper
• データソース種別:
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  • NDL
  • CiNii Articles