<原著>Percoll遠心分離法によるラット顎下腺導管細胞の単離  [in Japanese] <ORIGINAL>Isolation of rat submandibular ductal cells by Percoll centrifugation  [in Japanese]

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Author(s)

    • 根津 顕弘 NEZU Akihiro
    • 北海道医療大学歯学部歯科薬理学講座 Department of Dental Pharmacology, School of Dentistry, Health Sciences University of Hokkaido
    • 谷村 明彦 TANIMURA Akihiko
    • 北海道医療大学歯学部歯科薬理学講座 Department of Dental Pharmacology, School of Dentistry, Health Sciences University of Hokkaido
    • 東城 庸介 TOJYO Yosuke
    • 北海道医療大学歯学部歯科薬理学講座 Department of Dental Pharmacology, School of Dentistry, Health Sciences University of Hokkaido

Abstract

Ductal cells were purified by Percoll centrifugation after collagenase digestion of rat submandibular glands. After centrifugation, the cells were separated into two distinct fractions. A cell fraction obtained from the interphase of the centrifuge tube contained predominantly duct-like tubular structures, while the acini sedimented to the bottom. The ductal fraction was highly enriched in kallikrein, a ductal maker. To test the functional integrity of the prepared ductal cells, the effects of the membrane receptor agonists on intracellular free calcium concentration ([Ca^<2+>]i) were examined using the Ca^<2+> fluorescent indicator fura-2. Stimulation with carbachol, phenylephrine or isoproterenol increased significantly [Ca^<2+>]i in the ductal cells. These results indicate that the Percoll centrifugation may be used to isolate ductal cellsafter digestion of submandibular glands.

Journal

  • Higashi Nippon dental journal

    Higashi Nippon dental journal 19(1), 15-22, 2000-06-30

    Health Sciences University of Hokkaido

Codes

  • NII Article ID (NAID)
    110000409379
  • NII NACSIS-CAT ID (NCID)
    AN0008135X
  • Text Lang
    JPN
  • ISSN
    09109722
  • Data Source
    NII-ELS 
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