<原著>Percoll遠心分離法によるラット顎下腺導管細胞の単離 [in Japanese] <ORIGINAL>Isolation of rat submandibular ductal cells by Percoll centrifugation [in Japanese]
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Ductal cells were purified by Percoll centrifugation after collagenase digestion of rat submandibular glands. After centrifugation, the cells were separated into two distinct fractions. A cell fraction obtained from the interphase of the centrifuge tube contained predominantly duct-like tubular structures, while the acini sedimented to the bottom. The ductal fraction was highly enriched in kallikrein, a ductal maker. To test the functional integrity of the prepared ductal cells, the effects of the membrane receptor agonists on intracellular free calcium concentration ([Ca^<2+>]i) were examined using the Ca^<2+> fluorescent indicator fura-2. Stimulation with carbachol, phenylephrine or isoproterenol increased significantly [Ca^<2+>]i in the ductal cells. These results indicate that the Percoll centrifugation may be used to isolate ductal cellsafter digestion of submandibular glands.
- Higashi Nippon dental journal
Higashi Nippon dental journal 19(1), 15-22, 2000-06-30
Health Sciences University of Hokkaido