新しいアミノ酸脱水素酵素の開発 : 富山県の土壌からの検索(第 2 報) Screening for New Amino Acid Dehydrogenases : Soil of Toyama Prefecture as a Source of Microorganisms (II)
We screened for new NAD^+-dependent amino acid dehydrogenases, from soil of Toyama Prefecture. Various medium were used with a supplement of one of each of L-phenylalanine, L-lysine, L-tryptophan, L-isoleucine, or L-methionine. Some of the soil samples were preheated. Four hundred and fifty-eight strains were isolated from 120 soil samples. Fifty-six producers were found to have an activity of NAD^+-dependent amino acid dehydrogenase. Producers of isoleucine and leucine dehydrogenases were isolated and identified as Bacillus stearothermophilus and B. brevis, respectively. Microbacterium sp. was isolated and identified as a producer of phenylalanine dehydrogenase, by using a neutral medium containing L-methionine.
富山県立大学紀要 6, 101-106, 1996-03-29