投影X線顕微鏡の開発と生物学への応用

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  • Development of Projection X-Ray Microscopy and Its Biological Applications

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The biological applications of a projection x-ray microscope converted from a scanning electron microscope have been continued for neuronal tissue, HeLa cell, tooth etc using the imaging x-rays at the wavelength range of 0.5- 1 nm. Observations of HeLa cells cultivated on Formvar or SiN membrane and fixed with gultaraldehyde or zinc formalin at various phases of cell division cycle are reported here. Light microscpic and electron microscopic observations of the same field of the sample are made when necessary for mutual comparison. Morphological variety of inner structures are recognized in the spherical body at mitotic phase by x-ray microscopy. Structures observed in the reconstructed image of a confocal microscope by auto-fluorescence correspond to those by x-ray microscopy very well, which suggests the identification of mitochondria by the absorption contrast in the x-ray microscope image. Morphological difference of HeLa cells due to zinc formalin fixation is observed. A trial for improving the resolution of the projection x-ray microscopy deteriorated by Fresnel diffraction is performed by a method of image restoration.

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