投影X線顕微鏡によるウニの受精卵の内部構造の観察

• 矢田 慶治

  青森公立大学経営経済学部

• 篠原 邦夫

• 浜口 幸久

書誌事項

タイトル別名

• Observation of the Inner Structures of Sea-Urchin Eggs by ProjectionX-Ray Microscopy

抄録

The biological application of projection x-ray microscopy has been continued using the imaging x-rays at the wavelength range of 0.5-1 nm in order to ascertain whether the inner structures of the mitotic apparatus in sea-urchin eggs are detectable or not. As a result, it is found that spindle fibers, astral microtubules, chromosomes, and centrosomes of the mitotic apparatus are well recognized in the sample prepared by critical point drying of stabilized sea-urchin eggs using MEG buffer, though the structures of these mitotic apparatus are obscured to some extent by superposition of surface structures of the eggs, while no structures are recognized in the case of the natural drying. These structures are seen three dimentionally with stereoscopic pair. Image processing is successfully tried to improve the image resolution and contrast.

収録刊行物

• 青森公立大学紀要

  青森公立大学紀要 3 (2), 2-10, 1998-03-15

  青森公立大学

詳細情報

• CRID: 1571980076715039616
• NII論文ID: 110000485882
• NII書誌ID: AN10521710
• ISSN: 13419412
• 本文言語コード: ja
• データソース種別:
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