鶏の結直腸とRemak神経幹の抽出物中に存在する平滑筋抑制物質の性質〔英文〕  [in Japanese] Characterizations of Smooth Muscle Inhibitory Substances in Extracts of Chicken Rectum and Remak Nerve  [in Japanese]

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Author(s)

    • 小森 成一 KOMORI Seiichi
    • 岐阜大学農学部獣医学科家畜薬理学研究室 FACULTY OF AGRICULTURE GIFU UNIVERSITY, Laboratory of Veterinary Pharmacology
    • 松尾 呼野美 MATSUO Konomi
    • 岐阜大学農学部獣医学科家畜薬理学研究室 FACULTY OF AGRICULTURE GIFU UNIVERSITY, Laboratory of Veterinary Pharmacology

Abstract

鶏の結直腸の抽出物(ER)とRemak神経幹の抽出物(ERN)の両者には,アトロピン,メチセルジド及びピリラミンで処理したモルモットの回腸縦走筋に対して収縮作用に加えて弛緩作用も認められた。後者は,サブスタンスPで予め筋を収縮させて置くと明瞭に現れた。この抑制物質について検討した。254nmで紫外部吸収をモニターしながら,ERNのゲルろ過を行うと,弛緩活性は紫外部吸収の主ピークに一致した溶出旅画分に認められたので,ATP又はその関連物質による可能性について検討した。ATP,ADP.AMP及びAdenosineの中で,前二者にはERとERNに類似した弛緩作用があった。ATPなどがアルミナで吸着除去できることを確認した上で,ERとERNにアルミナ吸着操作を行うと,ERNの弛緩活性とUV吸収スペクトル上の254nmにおけるピークは共に消失した。ERの同活性は減弱した。加熱処理を1M塩酸液中で行うと,ERNとATPの弛緩活性は消失したが,ERの同活性は減弱されたに過ぎなかった。1Mの水酸化ナトリウム旅中では,ERの活性は減弱されたが,ERNとATPの活性はどちらも変わらなかった。ERの陽イオン交換クロマトを行うと,弛緩活性は三つの画分に分かれ,二つの画分の活性はキモトリプシン又はカルボキシペプチダーゼAとのインキュベーションにより失活した。以上の結果から,ERNの弛緩作用はATP又はその関連物質によること,ERの同作用には二つのペプチド様物質も関係していることが示された。

Acid-extracts of the chicken rectum (ER) and Remak nerve (ERN) caused a biphasic response of relaxation followed by a strong contraction, in the longitudinal muscle of the guinea-pig ileum (LMGPI) pretreated with atropine, methysergide and pyrilamine. The inhibitory effects on LMGPI were unaffected by tetrodotoxin, phentolamine and propranolol, and they were not mediated indirectly by stimulation of neural elements but directly by substance(s) other than catecholamines. In the gel filtration of ERN on Sephadex G-25, the elution curve of the relaxing activity was parallel to the elution curve of UV absorbance at 254 nm. ATP and ADP, but not AMP and adenosine, produced relaxation of LMGPI which resembled those produced by ERN and ER. Alumina treatment by which adenine nucleotides could be removed resulted in a total loss of the inhibitory activity of ERN associated with elimination of the peak at 254 nm on UV absorption spectra. This treatment was much less effective in reducing the inhibitory effect of ER. The inhibitory material in ERN, as ATP and ADP, was labile to boiling in 1 M HC1 but stable to boiling in 1 M NaOH. The inhibitory activity of ER was reduced to about 50% and 30% of the initial activity after boiling in 1 M HC1 and in 1 M NaOH, respectively. The ion exchange chromatography of ER on SP-Sephadex C-25 revealed the existence of three components responsible for the inhibitory activity, the first not adsorbed on the cation exchange resin, the second eluted at 0.2-0.4 M of the column buffer, and the third eluted at 1.0-1.5 M of the column buffer. The latter two components seemed to be pepsin-resistant but chymotrypsin- and carboxypeptidase A-susceptible. These results suggest that ATP or its closely-related compound may be the active principle for the inhibitory activity of ERN, and that two substances of peptide nature may also be responsible for the inhibitory activity of ER.

Journal

  • Research bulletin of the Faculty of Agriculture, Gifu University

    Research bulletin of the Faculty of Agriculture, Gifu University (50), p231-240, 1985-12

    Gifu University

Codes

  • NII Article ID (NAID)
    110000976487
  • NII NACSIS-CAT ID (NCID)
    AN00053423
  • Text Lang
    JPN
  • Article Type
    Departmental Bulletin Paper
  • Journal Type
    大学紀要
  • ISSN
    00724513
  • NDL Article ID
    3072811
  • NDL Source Classification
    RB89(畜産--家禽--鶏)
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産)
  • NDL Call No.
    Z18-181
  • Data Source
    NDL  NII-ELS  IR 
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