Application of Collagen Scaffolds for Hepatic Stem-Like Cell Transplantation APPLICATION OF COLLAGEN SCAFFOLDS FOR HEPATIC STEM-LIKE CELL TRANSPLANTATION

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Author(s)

    • WATANABE Go
    • Division of Gastroenterological Surgery, Department of Surgery, Akita University School of Medicine
    • ISHIKAWA Kiyoshi
    • Division of Molecular Cell Biology, Department of Anatomy and Biochemistry, Akita University School of Medicine
    • YOSHIKAWA Kiwamu
    • Division of Molecular Cell Biology, Department of Anatomy and Biochemistry, Akita University School of Medicine
    • HIRAYAMA Yasuhiro
    • Division of Molecular Cell Biology, Department of Anatomy and Biochemistry, Akita University School of Medicine
    • ENDO Erika
    • Division of Molecular Cell Biology, Department of Anatomy and Biochemistry, Akita University School of Medicine
    • KAMEDA Takashi
    • Division of Molecular Cell Biology, Department of Anatomy and Biochemistry, Akita University School of Medicine
    • LIU Yang
    • Division of Molecular Cell Biology, Department of Anatomy and Biochemistry, Akita University School of Medicine
    • SUGIYAMA Toshihiro
    • Division of Molecular Cell Biology, Department of Anatomy and Biochemistry, Akita University School of Medicine

Abstract

Tissue engineering using somatic stem cells is a new and exciting techniquewhich has the potential to facilitate tissue reconstruction. Recent studies on tissueengineering have indicated advantages in using collagen matrices to restitute the structureof an organ. Here we have demonstrated the efficacy of transplantation of hepaticstem-like (HSL) cells, a previously established liver epithelial cell line with potential fordifferentiation, using type I collagen sponge. To this end, HSL cells were cultured andtransplanted into Nagase's analbuminemic rat with spongy type I collagen matrices.Consequently, HSL was able to express both albumin and TAT after the culture incollagen sponge. In addition, the levels of serum albumin in recipient rats were foundto increase up to 1.5 fold relative to controls after transplantation. These findingssuggest that HSL cells are able to differentiate into functional hepatocytes, and thatbiodegradable collagen sponge may enhance hepatic differentiation of HSL cells byproviding an appropriate microenvironment.

Journal

  • Akita journal of medicine

    Akita journal of medicine 31(2), 113-119, 2004-09-21

    Akita University

References:  27

Codes

  • NII Article ID (NAID)
    110001046058
  • NII NACSIS-CAT ID (NCID)
    AN00009294
  • Text Lang
    ENG
  • Article Type
    ART
  • Journal Type
    大学紀要
  • ISSN
    03866106
  • NDL Article ID
    7110455
  • NDL Source Classification
    ZS7(科学技術--医学)
  • NDL Call No.
    Z19-945
  • Data Source
    CJP  NDL  NII-ELS  IR 
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