Streptococcus anginosus由来抗原によるマウス腹腔滲出細胞からのNO産生誘導機構  [in Japanese] Nitric oxide synthesis in marine peritoneal exudate cells stimulated with a Streptococcus anginosus antigen  [in Japanese]

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Author(s)

    • 山浦 千春 Yamaura Chiharu
    • 岩手医科大学歯学部口腔微生物学講座 Department of Oral Microbiology, School of Dentistry, Iwate Medical University

Abstract

Nitric oxide (NO) has been implicated in macrophage-mediated cytotoxicity against various pathogens and may play a role in persistent or latent infections. However, its overproduction induced by some bacterial antigens could cause damage to host tissues and cellular DNA. We have previously reported a novel bioactive antigen (SAA) from a culture supernatant of Streptococcus anginosus that induces NO synthesis by murine peritoneal exudate cells (PEC). In this study, I performed a further assessment of SAA-induced NO synthesis by PEC. The results indicated that SAA stimulated the macrophages in PEC as well as a murine macrophage cell line, J774.1, to produce NO with the accumulation of inducible NO synthase (iNOS) mRNA. SAA also stimulated the non-macrophage cells in PEC to produce IFN- γ, however, the endogenous IFN-γ was not involved in the SAA-induced NO synthesis and iNOS mRNA accumulation by the macrophages. Further, phosphorylation of both p38 and ERK1/2 mitogen-activated protein (MAP) kinase was observed in macrophages by the stimulation with SAA but p38 MAP kinase pathway could solely correlate with SAA-induced NO synthesis. Thus, the present results suggest that S. anginosus, by a bioactive antigen, SAA, could stimulate macrophages through p38 MAP kinase nathwav to induce NO svnthesis without heln of the endogenous TFN-γ.

Journal

  • Dental Journal of Iwate Medical University

    Dental Journal of Iwate Medical University 29(1), 3-14, 2004

    The Dental Society of Iwate Medical University

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