Enhancement of baculovirus lnfection in Spodoptera exigua(Lepidoptera:Noctuidae)larvae with Autographa californica nucleopolyhedrovirus or Nucotiana tabacum engineered with a granulovirus enhancin gene

DOI Web Site 被引用文献2件 参考文献41件
  • Hayakawa Tohru
    Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
  • Shimojo Ei-ichi
    Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
  • Mori Masashi
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
  • Kaido Masanori
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
  • Furusawa Iwao
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
  • Miyata Seiji
    Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
  • Sano Yoshitaka
    Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
  • Matsumoto Tsuguo
    Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
  • Hashimoto Yoshifumi
    Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
  • Granados Robert R.
    Boyce Thompson Institute for Plant Research at Cornell University

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タイトル別名
  • Enhancement of baculovirus infection in Spodoptera exigua (Lepidoptera: Noctuidae) larvae with Autographa californica nucleopolyhedrovirus or Nicotiana tabacum engineered with a granulovirus enhancin gene.

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A baculovirus virulence-associated protein, enhancin, was engineered for production in a baculovirus-insect cell expression system and used for transformation of tobacco plants. A recombinant Autographa californica nucleopolyhedrovirus (AcNPV) with the enhancin gene from Trichoplusia ni granulovirus, AcEnh26, was propagated in Sf9 cells. The infected cultured cells combined with either AcNPV occlusion bodies (OBs) or Spodoptera exigua NPV (SeNPV) OBs were fed to 3rd stadium larvae of S. exigua. Feeding larvae with AcEnh26-infected cells resulted in a 21-fold and 10-fold enhancement of infection by AcNPV and SeNPV, respectively, compared to controls. When a similar feeding assay was performed with engineered tobacco plants, a 10-fold enhancement of AcNPV infection was observed, but a marked enhancement of SeNPV infection was not observed. Thus, the engineering and expression of the baculovirus enhancin gene in homologous and heterologous organisms allowed us to analyze its effects on NPV infection of larvae which demonstrated its viral enhancement function.

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