15. Natural bovine bone morphogenetic protein (BMP) induces cytological and functional differentiation of preodontoblasts in vitro. :

NDLデジタルコレクション
  • Murata,Masaru
    Department of Oral Pathology, School of Dentistry, Okayama University
  • Tsujigiwa,Hidetsugu
    Department of Oral Pathology, School of Dentistry, Okayama University
  • Liu,Gui-ru
    Department of Oral Pathology, School of Dentistry, Okayama University
  • Nakano,Keisuke
    Department of Oral Pathology, School of Dentistry, Okayama University
  • Akagi,Takumi
    Department of Oral Pathology, School of Dentistry, Okayama University
  • Ruch,Jean Victor
    Institut de Biologie Medicale, Universite Louis Pasteur, Faculte de Medecine
  • Nagai,Noriyuki
    Department of Oral Pathology, School of Dentistry, Okayama University

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First lower molar dental papillae, isolated from day-17 mouse embryos, were embedded in 10 E l of BGJ-b medium including fetal calf serum and 0.5% agar. Two E1 of BMP solution at the different dose of BMP were added to the medium before getting. The papillae embedded in the semi-solidmedium were deposited on Millipore filters, and cultured for 6 days. This BMP fraction promoted functional differentiatioun of odontoblast-like cells and stimtulated the matrix secretion, dependent on the concentration of BMP.

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