Gene Expression of Bone Matrix Protein mRNA during BMP Induced Chondrogenesis and Osteogenesis by in situ Hybridization :

• NAGAI,Noriyuki

  Department of Oral Pathology, Okayama University Dental School

• NAGATSUKA,Hitoshi

  Department of Oral Pathology, Okayama University Dental School

• MURATA,Masaru

  Department of Oral Pathology, Okayama University Dental School

• INOUE,Masahisa

  Department of Oral Pathology, Okayama University Dental School

• AKAGI,Takumi

  Department of Oral Pathology, Okayama University Dental School

• QIN,Chun-Lin

  Department of Oral Pathology, Okayama University Dental School

• NAKANO,Keisuke

  Department of Oral Pathology, Okayama University Dental School

• ISIWARI,Yuzo

  Department of Oral Pathology, Okayama University Dental School

• KONOUCHI,Hironobu

  Department of Oral Pathology, Okayama University Dental School

• TSUJIGIWA,Hidetsugu

  Department of Oral Pathology, Okayama University Dental School

• CHIGONO,Yoshiho

  Department of Oral Surgery, Faculty of Medicine, Teikyo University

• TAKAGI,Tohru

  Department of Biochemistry, Tokyo Medical and Dental University Faculty of Dentistry

抄録

A sensitive in situ hybridization method with high resolution has been developed usin digoxigenin-II VTP labeled cRNA as a probe. The system was applied to a heterotopic induced bone with crude BMP. The localization of mRNA of the bone matrix proteins, osteopontin (OSP) and osteocalcin (OSC), was examined in the BMP-induced cartilage and bone in comparison with the endochondral ossification. In the heterotopic bone, OSP mRNA were detected in the osteoblast, young osteocyte and chondrocyte-like cells. In fatal endochondral ossification OSP mRNA was detected in the hypertrophic chondrocyte, osteoblast and young osteocyte, while OSC mRNA was detected in the osteoblast and young osteocyte, but hypertrophic chondrocyte was not detected. These results suggest that the chondrocyte-like cells in the BMP induced bone demonstrate the ambifunctions of the chondrocyte and osteoblast. Our results indicate that in situ hybridization immunohistochemistry is useful in identifying the cell phenotypes and their function.

収録刊行物

• Journal of hard tissue biology

  Journal of hard tissue biology 4 (1), 15-23,

  Japanese Society of Hard Tissue Research & Technology

キーワード

• BMP
• osteopontin
• osteonectin
• osteocalcin
• in situ hybridization
• nRNA

詳細情報

• CRID: 1540009770417455744
• NII論文ID: 110001143142
• NII書誌ID: AA11074332
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/11134407
• Web Site: https://search.jamas.or.jp/link/ui/1997001332
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles