アルブミン添加緩衝食塩水を用いた穿刺吸引細胞診の処理

DOI 被引用文献3件 参考文献13件

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  • Cellular preparation of the fine needle aspirates by means of albumin added phosphate buffered solution.

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Aspirates for aspiration biopsy cytology (ABC) are usually smeared on a glass slide, or collected in several rinsing solutions or fixatives and then automatically smeared by centrifugation. Physiologic saline solution (PS) is generally used as rinsing solution. When aspirates are stained by the Papanicolaou method, specimens after being rinsed in PS are paler than those directly smeared. Some of the cytoplasmic components in the aspirates seem to be dissolved by the PS. If they are rinsed in protein-rich fluid, cytological specimens may be stained well by the Papanicolaou method. This study evaluated 10 ABC specimens obtained from lung cancers shortly after lobectomy. PS containing 0.3%, 1.0%, or 3.0% albumin, pH 7.4 phosphate buffered saline (PBS) treated in the same manner as PS, and Sacomano's solution were used as rinsing solution. One, three, or six hours after collection in the solutions, the specimens were stained by the Papanicolaou method. The staining qualities of the specimens were compared with those of imprint specimens obtained at the same time as those for ABC. By the scoring method, we examined the specimens for the following five cytological factors: nuclear shape, chromatin pattern, and staining properties of the nucleus, nucleolus, and cystoplasm. Chromatin pattern and staining properties of the nucleus and cytoplasm were best in PBS with 3.0% albumin. Nuclear shape was well preserved in Sacomano's solution. In general, the scores of both PS and PBS increased with increasing albumin content. PBS with 3.0% albumin is thought to be the best rinsing solution within 3 hours.<BR>The results of this study show that PBS with 3.0% albumin to some extent can prevent a decrease in intensity of staining by the Papanicolaou method.

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