GELRITE培地によるアンモニア酸化細菌の純粋分離

• 徳山龍明

  日本大学農獣医学部農芸化学科

• 高橋 令二

  日本大学農獣医学部農芸化学科

書誌事項

タイトル別名

• On isolation of ammonia oxidizing bacteria from soil using Gelrite solidified plates.

抄録

An enrichment culture of soil ammonia oxidizing bacteria was filtrated through a 2.0 μm membrane filter (Nuclepore) to remove soil particles and filtrates were transferred to a fresh medium for the enrichment. An addition of antibiotics such as tetracycline and ampicillin and filtration through a 0.8 μm membrane filter were useful to decrease contamitants. Using a higher enrichment culture colonies of ammonia oxidizing bacteria (300-600μm in diameter) were obtained on GELRITE-solidified plates. Cells picked up from the colony by means of capillary tubes were grown on a liquid media and produced nitrite from ammonia but did not show heterotrophic growth. Such isolation of ammonia oxidizing bacteria in culture using GELRITE-solidified plate was accomplished with comparative ease.

収録刊行物

• Bulletin of Japanese Society of Microbial Ecology

  Bulletin of Japanese Society of Microbial Ecology 4 (2), 101-105, 1989

  日本微生物生態学会

キーワード

• Ammonia oxidizing bacteria
• Gelrite plate
• bacterial isolation

詳細情報

• CRID: 1570572701869557376
• NII論文ID: 110001269752
• NII書誌ID: AN10424816
• ISSN: 09117830
• 本文言語コード: ja
• データソース種別:
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