Differentiation of Thermophilic Anaerobic Gram-positive Bacteria by Random Amplified Polymorphic DNA Analysis

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Author(s)

Abstract

Random amplified polymorphic DNA (RAPD) analysis was applied to the differentiation of 11 thermophilic clostridia species, 11 type strains and 14 reference strains, at the species level. The 6 primers used in amplification generated 2 to 11 bands with 170-3,300 bp that were different depending on each of the strains and the primers used. From a comparison of RAPD fingerprints: sizes, numbers, and intensities of bands, it was demonstrated that (<i>Clostridium</i>) <i>thermocellum</i>, (<i>Clostridium</i>) <i>thermolacticum</i>, <i>Moorella thermoacetica</i>, and <i>Thermoanaerobacterium thermosaccharolyticum</i> were genotypically homogeneous at each species level. Each species showed species-specific and -common bands in RAPD profiles that were most pertinent for the discrimination of strains at the species level. In contrast, it was shown that each species of <i>Thermoanaerobacter thermohydrosulfuricus</i>, <i>Thermoanaerobacter thermocopriae</i> and <i>Thermoanaerobacterium thermosulfurigenes</i> was genotypically heterogeneous because none of the 6 primers produced species-common band patterns. However, each type strain of thermophilic clostridia species gave different RAPD fingerprints with each primer. Results obtained by the RAPD analysis therefore complement and support the current classification of these species and this study also shows that RAPD has the potential to differentiate between these species.

Journal

  • Microbes and environments

    Microbes and environments 16(2), 91-99., 2001-06-01

    Japanese Society of Microbial Ecology

References:  23

Codes

  • NII Article ID (NAID)
    110001271023
  • NII NACSIS-CAT ID (NCID)
    AA11173196
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13426311
  • NDL Article ID
    5823746
  • NDL Source Classification
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL Call No.
    Z54-J644
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE 
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