Isolation and Characterization of Heterotrophic Chemoorganotrophic Bacteria from the Roots of Lepironia articulata (reedgrass) Grown on Strongly Acidic Sulfate Soil in South Thailand.

• OISHI RYUYA

  Department of Human Environmental Science, Faculty of Agriculture, Shizuoka University Present adress: Asian Natural Environmental Center, The University of Tokyo

• NIOH ICHIO

  NODAI Research Institute, Tokyo University of Agriculture

• HAYATSU MASAHITO

  Department of Human Environmental Science, Faculty of Agriculture, Shizuoka University

• NIOH TAKAKO

  Ichimura Junior College

• LIMTONG PITAYAKON

  Division of Soil and Water Conservation, Department of Land Development

抄録

Bacterial communities inhabiting the rhizosphere of Lepironia articulata (reedgrass) grown in a strongly acidic sulfate soil distributed in the province of Narathiwat, Thailand were investigated. Fresh roots of L. articulata were cut from the subterranean stem, and the bacteria were isolated from the homogenate on dilution plates of glucose-yeast extract agar medium adjusted to pH 4.5. In or on the roots, 3.3×10⁷ bacteria(cfu)g^-1 fresh roots were present. From the dilution plates, 60 bacteria were randomly isolated and their characteristics were examined. All the isolates were Gram-negative rods and grew well at pH 4.5. Analysis of cell fatty acids and the restriction fragment length polymorphism (RFLP) of PCR-amplified 16S rRNA genes indicated that the isolates could be divided into 4 groups (Group I to IV). Group I (65% of total) isolates failed to grow on the agar plate at pH 7. The sequence analysis of 16S rRNA genes of a Group I isolate showed that it resembled Acidocella facilis with 98.5% homology. Growth of Group I bacteria was suppressed by acetate. The next most abundant bacteria, belonging to Group II, grew at pH 7.0 and belonged to Burkholderia. These bacteria accounted for 28% of all the isolates. Bacteria of Group III and IV (identified as Bacillus subtilis) were a minor constituent only. Group III isolates were similar to the genus Xanthomonas, but the similarity of the 16S rRNA gene base arrangement with the nearest species was below 90%.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 14 (2), 69-75, 1999

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• rhizosphere
• <i>Lepironia articulata</i>
• acid sulfate soil
• acidophilic bacteria
• <i>Acidocella</i>

詳細情報

• CRID: 1390001204344688896
• NII論文ID: 110001271107
• NII書誌ID: AA11173196
• DOI: 10.1264/jsme2.14.69
• ISSN: 13474405
• ISSN: 13426311
• ISSN: http://id.crossref.org/issn/13426311
• NDL書誌ID: 4785214
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4785214
• Web Site: http://www.jstage.jst.go.jp/article/microbes1996/14/2/14_2_69/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可