Protein Phosphatase Inhibition Assay for Detection of Microcystins in Lake Water and Microcystis Cultures.

• YAO YASUKO

  Applied Technology Research Center, NKK Corporation

• UJIIE YUMI

  Analytical Center, Kokan Keisoku Service

• WATANABE MASAYUKI

  Department of Botany, National Science Museum

• YAGI OSAMI

  Water and Soil Environment Division, The National Institute for Environmental Studies

• TAKAMURA YOSHICHIKA

  Department of Bioresource Science, Faculty of Agriculture, Ibaraki University

書誌事項

タイトル別名

• Protein Phosphatase Inhibition Assay fo

抄録

The protein phosphatase inhibition assay was used to quantify low levels of microcystin in lake water polluted by cyanobacterial blooms of Microcystis, and toxin concentrations in cultures of 15 strains of Microcystis. This method was highly sensitive permitting measurement of microcystin in concentrations of the order of μg1^-1. The concentrations of microcystin in the surface water of Lake Kasumigaura in the summer of 1995 were in the range of 0.16 to 2.7μg1^-1 microcystin-LR equivalents.<br>On the other hand, markedly high levels of microcystin (160 to 1, 429μg1^-1) were measured in the culture fluids of M. aeruginosa TAC 192-2, M. ichthyoblabe TAC 69, M. ichthyoblabe TAC 113-1, M. viridis TAC 45-1, M. viridis TAC 64 and M. viridis TAC 92. There were strains which did not produce microcystin in the culture fluid within the same species of Microcystis. Therefore, the toxin productivity in the culture fluid is not a useful criterion for discrimination of the species of Microcystis.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 13 (3), 149-157, 1998

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• Plant Science
• Soil Science
• General Medicine
• Ecology, Evolution, Behavior and Systematics

詳細情報

• CRID: 1390282679320991616
• NII論文ID: 110001272821
• NII書誌ID: AA11173196
• DOI: 10.1264/jsme2.13.149
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 4583048
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4583048
• Web Site: http://www.jstage.jst.go.jp/article/microbes1996/13/3/13_3_149/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可