大腸癌におけるｐ５３変異，ｐ２１｀（ＷＡＦ１）´発現とアポトーシス誘導の検討

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  • Relationship between p53, p21 and Apoptosis in Colorectal Cancer.

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In 92 colon cancer patients, point mutation of the p53 gene and expression of the p21 gene were determined to discuss about appearance of apoptosis. Point mutation of the p53 gene was determined by PCR-SSCP. p21 expression was determined positive upon immunostaining when more than 20% of the nucleus in cancer cells were stained. Apoptosis was detected by DNA ladder sequencing. No correlation was noted between the presence of p53 gene mutation, expression of p21 gene and pathological factors, nor was correlation noted between p53 gene mutation and p21 gene staining rate, by apperance of DNA ladder. However a certain trend was observed in those cells with p21 positive staining showing the least appearance of DNA ladder (p<0.0115). Among those without p53 gene mutation, DNA ladder did not appear when those p21 staining was positive, and it was noted much when p21 staining was negative (p<0.0015). From the above findings, it is known that in the presence of the p21 gene DNA in repaired and induces no apoptosis, and that when the p53 gene functions normally, such a trend is further accelerated by the p21 gene.

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