疲労と脳内サイトカイン

• 片渕 俊彦

  九州大学大学院医学研究院統合生理学教室

• 武 幸子

  九州大学大学院医学研究院統合生理学教室

書誌事項

タイトル別名

• Fatigue and Brain Cytokines
• ヒロウ ト ノウナイ サイトカイン

抄録

The immunologically induced fatigue was conducted in rats by intraperitoneal (IP) injection of a synthetic double-stranded RNAs, poly I : C. An IP injection of poly I : C (3mg/kg) decreased the daily amounts of spontaneous running wheel activity to about 40-60% of the preinjection level until day 9, then gradually returned to the baseline level by day 14. In the open field test, the locomotor activity of the poly I : C-injected rats decreased on day 1, but it was not different from the saline-injected group on day 7, suggesting that the poly I : C-induced fatigue on day 7 was not due to the peripheral problems such as muscle/joint pain, but involved the central nervous system. Quantitative analysis of mRNA levels using a real-time capillary reverse transcriptase (RT)-PCR method revealed that the amount of mRNA for interferon-c! (IFN-α) increased in the medial preoptic, paraventricular, ventromedial hypothalamic nuclei, and cortex on day I and even on day 7. Serotonin transporter (5-HTT) mRNA, which is known to be induced by IFN-α in astrocytes, also increased in the same brain regions on day 7. Interleukin-1β (IL-1β) and inhibitor of nuclear factor κB (I-κB) mRNAs increased on day 1, but recovered within a week. In vivo brain microdialysis revealed that the extracellular concentration of 5-HT in the prefrontal cortex decreased after poly I : C injection. Since this decrease in 5-HT was blocked by a selective 5-HT reuptake inhlbitor, fluoxetine, it was thought to be due to an enhanced expression of 5-HTT. Furthermore, the poly I : C-induced suppression of the running wheel activity was attenuated by a 5-HT_<1A> receptor agonist, 8-hydroxy-2-(di-n-propylamino) tetraline (8-0HDPAT). Finally, the poly I : C-induced decrease in activity was conditioned using poly I : C (IP) as an unconditioned stimulus and saccharin drinking as a conditioned stimulus, and this conditioned response was completely blocked by 8-0H-DPAT, indicating the presence of neuronal circuit for the central fatigue. These findings, taken together, suggest that brain IFN-α and 5-HT system play an important role in the neuronal mechanisms of the immunologically induced fatigue by poly I : C.

収録刊行物

• 心身医学

  心身医学 45 (4), 265-273, 2005

  一般社団法人 日本心身医学会

キーワード

• 免疫学的疲労
• poly I:Cインターフェロン-α
• セロトニントランスポーター
• 古典的条件づけ

詳細情報

• CRID: 1390001204891164288
• NII論文ID: 110001523462
• NII書誌ID: AN00121636
• DOI: 10.15064/jjpm.45.4_265
• ISSN: 21895996
• ISSN: 03850307
• NDL書誌ID: 7296866
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7296866
• Web Site: https://search.jamas.or.jp/link/ui/2005150764
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可