イネいもち病菌エリシターで刺激したイネ葉身細胞において作動する膜情報伝達系. II. カルシウム調節タンパク質の関与

DOI NDLデジタルコレクション Web Site Web Site 被引用文献5件
  • 加納 大聖
    Department of Agricultural Chemistry, Faculty of Agriculture, Tamagawa University Yokohama Research Center, Chisso Corp.
  • 芳賀 実
    Department of Agricultural Chemistry, Faculty of Agriculture, Tamagawa University
  • 関沢 泰治
    Department of Agricultural Chemistry, Faculty of Agriculture, Tamagawa University

書誌事項

タイトル別名
  • Transmembrane Signalling Operated at Rice Blade Cells Stimulated by Blast Fungus Elicitor II. Participation of Calcium Modulated Protein
  • イネいもち病菌エリシターで刺激したイネ葉身細胞において作動する膜情報伝達系-2-カルシウム調節タンパク質の関与〔英文〕
  • イネ イモチビョウキン エリシター デ シゲキシタ イネ ヨウシン サイボウ
  • Causal Analysis of Reaction Cascades in the Induced Defense Mechanisms of Rice Plants (Part XIII)

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抄録

The effects of molecular probes on the dynamic behaviours of O-·2 generation and α-linolenate release from rice (Oryza sativa) blade tissue preparations with press-injured spot (2mm), pre-treated with the molecular probes and post-stimulated with blast fungus (Pyricularia oryzae) elicitor, were further surveyed. Compound W-7 or ophiobolin A, potent inhibitors on the function of CaM (calmodulin), strongly inhibited both O-·2 generation at 10μM and α-linolenate release at 500μM after the elicitor stimulation. The comparative coupledenzymic analysis on the behaviour of free CaM in the healthy or infected rice blades with blast fungus conidia revealed an immediate decrease of free CaM by the infection. TPA (10μM), an agonist of diacylglycerol for proteinkinase C, did not activate both parameters with the elicitor stimulation, although TPA (10μM) markedly activated the α-linolenate release at the later phase with the stimulation. The application of H-7 (1mM) or staurosporin (10μM), unspecific inhibitors on proteinkinases, inhibited the O-·2 generation, but staurosporin (50μM) activated the α-linolenate release at the later phase and H-7 (1mM) inhibited the α-linolenate release after the elicitor stimulation. The application of 1, 2-benzisothiazol-3(2H)-one 1, 1-dioxide (1mM) with the elicitor stimulation further activated the O-·2 generation and markedly activated the α-linolenate release at the later phase. The lines of evidence strongly bore out the suggestion that the formations of Ca2+-MPs (Ca2+-calcium modulated proteins), which signal-coupled with the operation of phospholipase C system, play an indispensable role in activating rice blade O-·2 forming redox system and phospholipase A2.

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