Expression of a Single-Chain Antibody against GA_<24/19> in Vascular Tissues Induces Dwarf Phenotype for Rice Plants

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Author(s)

    • Tanaka Yoko TANAKA Yoko
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • Fukuda Akari FUKUDA Akari
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • FUJIWARA Toru
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • SUZUKI Yoshihito
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • YAMAGUCHI Isomaro
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • YONEYAMA Tadakatsu
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • HAYASHI Hiroaki
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Abstract

GA_1 and GA_4 are active gibberellins (GAs) in rice plants, which are synthesized from GA_<19> and GA_<24>, respectively. However, the plant tissues where active GAs biosynthesis occurs and the route of their transport have not been fully elucidated. To study the importance of vascular tissues for the function of GAs, cDNA encoding anti-GA_<24/19> single-chain variable fragment (scFv) was expressed under the control of the thioredoxin h promoter, which is active in companion cells of phloem tissues. The transgenic rice plants showed a dwarf phenotype with increased levels of GA_<19>. This indicated that the expression of the anti-GA_<24/19> scFv antibody in companion cells changed the synthesis and/or translocation of GAs in rice plants effectively, suggesting that the vascular tissues play an important role in the function of GAs in rice plants.

Journal

  • Soil Science and Plant Nutrition

    Soil Science and Plant Nutrition 50(8), 1281-1285, 2004-12-01

    Japanese Society of Soil Science and Plant Nutrition

References:  21

Cited by:  1

Codes

  • NII Article ID (NAID)
    110001720413
  • NII NACSIS-CAT ID (NCID)
    AA00844314
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00380768
  • NDL Article ID
    7200519
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産)
  • NDL Call No.
    Z53-G349
  • Data Source
    CJP  CJPref  NDL  NII-ELS  Crossref 
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