PCR-RFLP image analysis: a practical method for estimating isolate-specific allele frequency in a population consisting of two different strains of the pinewood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelencoididae)

• Takemoto Shuhei

  Graduate School of Agriculture, Kyoto University

• Kanzaki Natsumi

  Faculty of Agriculture, Kagoshima University

• Futai Kazuyoshi

  Graduate School of Agriculture, Kyoto University

抄録

A practical method for estimating the allele frequency in a population of the pinewood nematode, Bursaphelenchus xylophilus, was developed by employing the technique of PCR-RFLP image analysis, in which a relative quantity of DNA templates is determined. At first, nematode individuals of two isolates, a virulent (S10) and an avirulent isolate (C14-5), were mixed at various proportions, and then PCR-RFLP patterns of the heat-shock protein 70A (hsp70A) gene fragment were obtained from each mixture. Regression analysis between logarithmic values of relative intensity of isolate-specific bands and those of a relative number of individuals of the two strains showed a strong linear relationship. These regression lines were applied to pursue temporal changes in the frequency of the hsp70A alleles in several experimental populations. The changes observed in allele frequencies were well explained with an overdominance model.

収録刊行物

• Ａｐｐｌｉｅｄ　Ｅｎｔｏｍｏｌｏｇｙ　ａｎｄ　Ｚｏｏｌｏｇｙ

  Ａｐｐｌｉｅｄ　Ｅｎｔｏｍｏｌｏｇｙ　ａｎｄ　Ｚｏｏｌｏｇｙ 40 (3), 529-535, 2005

  日本応用動物昆虫学会

キーワード

• intraspecific competition
• stabilizing selection
• replacement series

詳細情報

• CRID: 1390282681220210816
• NII論文ID: 110001793488
• NII書誌ID: AA00543238
• DOI: 10.1303/aez.2005.529
• ISSN: 1347605X
• ISSN: 00036862
• NDL書誌ID: 7404465
• Web Site: http://id.ndl.go.jp/bib/7404465
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7404465
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可