Expression of Corticotropin Releasing Factor (CRF), Urocortin and CRF Type 1 Receptors in Hypothalamic-Hypophyseal Systems Under Osmotic Stimulation

• Imaki T.

  Department of Bioregulation, Institute of Gerontology, Nippon Medical School

• Katsumata H.

  Department of Bioregulation, Institute of Gerontology, Nippon Medical School

• Miyata M.

  Department of Physiology, Tokyo Women's Medical University Laboratory for Learning and Memory, Brain Science Institute

• Naruse M.

  Department of Medicine, Tokyo Women's Medical University

• Imaki J.

  Department of Anatomy, Nippon Medical School

• Minami S.

  Department of Bioregulation, Institute of Gerontology, Nippon Medical School

抄録

The expression of corticotropin releasing factor (CRF) and urocortin in hypothalamic magnocellular neurones increases in response to osmotic challenge. To gain a better understanding of the physiological roles of CRF and urocortin in fluid homeostasis, CRF, urocortin and CRF type 1 receptor (CRFR-1) gene expression was examined in the hypothalamic-hypophyseal system using in situ and double-label in situ hybridization following chronic salt loading. CRFR-1 expression was further examined by immunohistochemistry and receptor binding. Ingestion of hypertonic saline by Sprague-Dawley rats for 7 days induced CRF mRNA exclusively in the oxytocin neurones of the magnocellular paraventricular nucleus (PVN) and the supraoptic nucleus (SON), but induced CRFR-1 mRNA in both oxytocin and vasopressin-containing magnocellular neurones. Hypertonic saline treatment also increased urocortin mRNA expression in the PVN and the SON. In the SON, urocortin was localized to vasopressin and oxytocin neurones but was rarely seen in CRF-positive cells. Changes in CRFR-1 mRNA expression in magnocellular neurones by hypertonic saline treatment were accompanied by changes in CRFR-1 protein levels and receptor binding. Hypertonic saline treatment increased CRFR-1-like immunoreactivity in the magnocellular PVN and SON, and decreased it in the parvocellular PVN. CRF receptor binding in the PVN and SON was also increased in response to osmotic stimulation. Finally, hypertonic saline treatment increased CRFR-1 mRNA, CRFR-1-like immunoreactivity and CRF receptor binding in the intermediate pituitary. These results demonstrate that the increase in the expression of CRF and urocortin message in magnocellular neurones induced by salt loading is accompanied by an increase in CRF receptor levels and binding in the hypothalamus and intermediate pituitary. Thus, CRF and urocortin may exert modulatory effects locally within magnocellular neurones as well as at the pituitary gland in response to osmotic stimulation.

収録刊行物

• 日本医科大学老人病研究所紀要

  日本医科大学老人病研究所紀要 8 87-97, 2002-03-25

  日本医科大学

キーワード

• CRF
• CRF receptor
• urocortin
• magnocellular neurones
• osmotic stimulation
• oxytocin
• vasopressin
• pituitary gland

詳細情報

• CRID: 1571135651874931072
• NII論文ID: 110001798191
• NII書誌ID: AN1047681X
• ISSN: 13409662
• 本文言語コード: en
• データソース種別:
  • CiNii Articles