MITE-transposon Display Efficiently Detects Polymorphisms among the Oryza AA-genome Species

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Author(s)

Abstract

Miniature inverted repeat transposable elements (MITEs) are a major component of interspersed repetitive sequences in the rice genome. These elements serve as excellent tools for fine genomic analysis throughout the genome. Using the rice genome database, we evaluated MITEs present in a 200-kb region surrounding the rice waxy locus and selected four MITE subfamilies for MITE-transposon display (MITE-TD) analyses. MITE-TD, which is an AFLP-related technique based on MITE sequences, was applied to detect polymorphisms among the AA-genome Oryza species using the four selected MITEs. The MITE-TD used here enabled the most efficient detection of polymorphisms of all the molecular marker techniques applied to date in the Oryza species. Of the four MITEs, Mashu, a new MITE family, was found to be the best system fo detecting the polymorphisms, with a detection frequency 3-1.5 times higher than that of the other three MITEs. The MITE-TDs also revealed information about genetic variations within the AA-genome species, and the complexity of the genetic relationships between O. sativa and O. rufipogon.

Journal

  • Breeding Science

    Breeding Science 53(2), 125-132, 2003-06-01

    Japanese Society of Breeding

References:  29

Cited by:  4

Codes

  • NII Article ID (NAID)
    110001803227
  • NII NACSIS-CAT ID (NCID)
    AA11353132
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13447610
  • NDL Article ID
    6545645
  • NDL Source Classification
    ZR6(科学技術--農林水産)
  • NDL Call No.
    Z54-J372
  • Data Source
    CJP  CJPref  NDL  NII-ELS 
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