Efficient Transgene Expression in Chrysanthemum, Dendranthema grandiflorum (Ramat.) Kitamura, by Using the Promoter of a Gene for Chrysanthemum Chlorophyll-a/b-binding Protein

• Aida Ryutaro

  National Institute of Floricultural Science

• Ohira Kazuyuki

  Institute for Advanced Technology, Suntory Ltd. Present address: Intellectual Property Devision, Nara Institute of Science and Technology

• Tanaka Yoshikazu

  Institute for Advanced Technology, Suntory Ltd.

• Yoshida Kazuya

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Kishimoto Sanae

  National Institute of Floricultural Science

• Shibata Michio

  National Institute of Floricultural Science

• Ohmiya Akemi

  National Institute of Floricultural Science

抄録

We investigated the usefulness of the promoter of a gene for chrysanthemum chlorophyll-a/b-binding protein (Cab) for transgene expression in the chrysanthemum Dendranthema grandiflorum (Ramat.) Kitamura. We used the promoter region of a Cab gene isolated from the chrysanthemum-wild species D. japonicum Makino. The 35S promoter of cauliflower mosaic virus (CaMV) or the Cab promoter was fused to the β-glucuronidase gene (gus) and introduced into the chrysanthemum. We obtained 300 putative transformants (115 with 35S/gus and 185 with Cab/gus). GUS assay of the leaves of the in vitro plants revealed that 9.6 % (11/115) of the putative plants to which 35S/gus had been introduced and 24.3 % (45/185) of the putative plants to which Cab/gus had been introduced were GUS-positive. Southern blot analysis showed that even the GUS-negative plants harbored the gus gene in their genomes. The Cab promoter expressed the transgene more efficiently than the 35S promoter and could be used for transgene expression in chrysanthemum leaf tissues.<br>

収録刊行物

• Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ

  Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ 54 (1), 51-58, 2004

  日本育種学会

キーワード

• <i>Dendranthema grandiflorum</i>
• chrysanthemum
• transformation
• chlorophyll <i>a</i>/<i>b</i> binding protein gene
• promoter

詳細情報

• CRID: 1390282679698502784
• NII論文ID: 130004145945
• NII論文ID: 110001803265
• NII書誌ID: AA11317194
• DOI: 10.1270/jsbbs.54.51
• COI: 1:CAS:528:DC%2BD2cXktlCjtL4%3D
• ISSN: 13473735
• ISSN: 13447610
• NDL書誌ID: 6866806
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6866806
• Web Site: http://www.jstage.jst.go.jp/article/jsbbs/54/1/54_1_51/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可