Marker-assisted Analysis for Soybean Hard Seededness with Isozyme and Simple Sequence Repeat Loci

• Sakamoto Shin-ichi

  Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University

• Abe Jun

  Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University

• Kanazawa Akira

  Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University

• Shimamoto Yoshiya

  Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University

Bibliographic Information

Other Title

• Marker-assisted analysis for hard seededness with isozyme and simple sequence repeat loci

Abstract

We determined the genomic regions of wild soybean (Glycine max subsp. soja) carrying quantitative trait loci (QTL) for hard seededness, a distinct character between the wild and cultivated soybeans. The segregation of hard seededness, as evaluated using seed coat permeability (SCP), was examined in the progeny of a cross between wild and cultivated soybeans. The high parent-offspring correlation coefficient between F₂ and F₃ indicated that SCP was a highly heritable character. A marker-assisted analysis of five isozyme and 131 simple sequence repeat (SSR) loci revealed that at least two major and one minor QTL were involved in controlling the trait. The QTL near an SSR marker (Satt459) in molecular linkage group D1b + W had the greatest effect on SCP, and accounted for 23.8 and 38.5 % of the phenotypic variance observed in the F₂ and F₃ mean, respectively. Seed coat color, determined by a locus inhibiting seed coat color (I) and a pubescence color locus (T), was also closely associated with SCP in F₄ to F₆, although seed coat color did not appear to be associated with SCP in the F₂. The results of this and a previous study suggest that different sets of QTL contribute to the phenotypic difference of seed coat permeability in wild and cultivated soybeans to different degrees.<br>

Journal

• Breeding Science

  Breeding Science 54 (2), 133-139, 2004

  Japanese Society of Breeding

Keywords

• <i>Glycine max</i>
• hard seededness
• soybean
• quantitative trait loci
• isozyme
• simple sequence repeat
• domestication syndrome

Details

• CRID: 1390001204723751680
• NII Article ID: 130004056995
• NII Article ID: 110001803277
• NII Book ID: AA11317194
• DOI: 10.1270/jsbbs.54.133
• COI: 1:CAS:528:DC%2BD2cXntVGqt7Y%3D
• ISSN: 13473735
• ISSN: 13447610
• NDL BIB ID: 6955517
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6955517
• Web Site: http://www.jstage.jst.go.jp/article/jsbbs/54/2/54_2_133/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed