Cloning and Characterization of a cDNA Encoding 1 - aminocyclopropane - 1 - carboxylate (ACC) Synthase (PPACS3) from Ripening Fruit of Japanese pear (Pyrus pyrifolia Nakai)

ニホンナシ成熟果実由来ACC合成酵素cDNA(PPACS3)のクローニングと発現解析 Cloning and Characterization of a cDNA Encoding 1 - aminocyclopropane - 1 - carboxylate (ACC) Synthase (PPACS3) from Ripening Fruit of Japanese pear (Pyrus pyrifolia Nakai)

- 板井章浩 ITAI Akihiro
- 鳥取大学農学部 Laboratory of Horticultural Science, Faculty of Agriculture, Tottori University
- 田辺賢二 TANABE Kenji
- 鳥取大学農学部 Laboratory of Horticultural Science, Faculty of Agriculture, Tottori University
- 田村文男 [他] TAMURA Fumio
- 鳥取大学農学部 Laboratory of Horticultural Science, Faculty of Agriculture, Tottori University
- 友光政雄 TOMOMITSU Masao
- 鳥取大学農学部 Laboratory of Horticultural Science, Faculty of Agriculture, Tottori University

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Author(s)

- 板井章浩 ITAI Akihiro
- 鳥取大学農学部 Laboratory of Horticultural Science, Faculty of Agriculture, Tottori University
- 田辺賢二 TANABE Kenji
- 鳥取大学農学部 Laboratory of Horticultural Science, Faculty of Agriculture, Tottori University
- 田村文男 [他] TAMURA Fumio
- 鳥取大学農学部 Laboratory of Horticultural Science, Faculty of Agriculture, Tottori University
- 友光政雄 TOMOMITSU Masao
- 鳥取大学農学部 Laboratory of Horticultural Science, Faculty of Agriculture, Tottori University

Abstract

ニホンナシ品種'二十世紀'成熟果実よりACC合成酵素をコードするcDNAクローン(PPACS3)を単離した.推定されるタンパク質の分子量は55.5kDaで,495アミノ酸残基からコードされていた.推定アミノ酸は他の植物由来のACC合成酵素でよく保存されている7つの領域を含み,ニホンナシ果実由来のACC合成酵素(PPACS1,2)の推定アミノ酸配列と58%,55%の相同性を示した.ノーザンブロット及びRT -PCR解析の結果,PPACS3遺伝子は成熟果実で非常に低いレベルで発現しており,また供試したすべての品種の成熟果実で発現していた.さらに成熟果実に傷実処理を与えることにより,発現は著しく増大した.これらのことからPPACS3遺伝子は,成熟及び傷害の両方のエチレン生成に関与するものと思われた.

We have isolated a cDNA (PPACS3) encoding ACC synthase from the ripening fruit of the Japanese pear (Pyrus pyrifolia Nakai cv. Nijisseiki). This cDNA clone consisted of 1891 bp, containing an open reading frame of 1485 bp that coded for a polypeptide with 495 amino acid residues. The deduced amino acids of PPACS3 revealed 58% and 53% identity to those of PPACS1 and PPACS2, respectively. Northern blot and RT-PCR analyses showed that the PPACS3 mRNA was expressed at a low level in ripening fruits in all cultivars in Japanese pear. Moreover, wound treatment increased the expression of PPACS3 mRNA, indicating that the PPACS3 gene is associated with fruit ripening and wounding.

Journal

Journal of the Japanese Society for Horticultural Science

Journal of the Japanese Society for Horticultural Science 72(2), 99-106, 2003-03-15

THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE

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ニホンナシ成熟果実由来ACC合成酵素cDNA(PPACS3)のクローニングと発現解析 Cloning and Characterization of a cDNA Encoding 1 - aminocyclopropane - 1 - carboxylate (ACC) Synthase (PPACS3) from Ripening Fruit of Japanese pear (Pyrus pyrifolia Nakai)

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