蛍光ＡＦＬＰ法によるワケギ栽培系統の識別

• 荒木 直幸

• 古田 貴音

• 小原 隆由

• 山内 直樹

• 執行 正義

書誌事項

タイトル別名

• Cultivar Discrimination among Wakegi Onion (Allium * wakegi Araki) by Fluorescent AFLP Technique
• ケイコウ AFLPホウ ニ ヨル ワケギ サイバイ ケイトウ ノ シキベツ

抄録

To identify cultivars of wakegi onion (Allium X wakegi Araki), nine virus-free clones (Hiroshima 1 go-Hiroshima 9 go) were examined by fluorescent AFLP (Amplified Fragment Length Poly-morphism) technique, using 16 primer combinations. Twelve out of the 16 primer combinations amplified a total of 678 DNA fragments (peaks) in nine wakegi onion clones. Eight primer combinations produced 11 polymorphic markers available for discriminating the nine clones from each other, except between Hiroshima 1 go ('Shimonoseki') and Hiroshima 2 go ('Kanshirazu'). The AFLP data revealed that 26.3% and 23.5% of the 678 DNA fragments were derived from Japanese bunching onion and shallot, respectively. Five out of 11 polymorphic markers, were seemingly originated from Japanese bunching onion, whereas three from shallot. These results verified that wakegi onion are interspecific hybrids whose parents are the Japanese bunching onion and shallot. Fluorescent AFLP technique is a useful method for discriminating wakegi onion cultivars.

収録刊行物

• 園芸学会雑誌

  園芸学会雑誌 72 (3), 230-235, 2003

  一般社団法人　園芸学会

キーワード

• Allium
• Cultivar identification
• DNA marker
• Fluorescent AFLP
• Wakegi onion

詳細情報

• CRID: 1390282681279266944
• NII論文ID: 110001803425
• NII書誌ID: AN00025257
• DOI: 10.2503/jjshs.72.230
• COI: 1:CAS:528:DC%2BD3sXkt12isbk%3D
• ISSN: 1880358X
• ISSN: 00137626
• ISSN: http://id.crossref.org/issn/00137626
• NDL書誌ID: 6587994
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010670989
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6587994
• Web Site: http://www.jstage.jst.go.jp/article/jjshs1925/72/3/72_3_230/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10470308
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • NDL-Digital
  • CiNii Articles
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