アミプロフォスメチルおよびコルヒチンによるビワ四倍体の作出  [in Japanese] Induction of Tetraploid in Loquat with Amiprofos-methyl and Colchicine  [in Japanese]

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Abstract

三倍体ビワを利用した無種子品種育成のための育種材料となる四倍体ビワの作出を,アミプロホスメチル(APM)およびコルヒチンの2薬剤を用いて,いくつかの処理方法により試みた.その結果,APMの処理により4個体,コルヒチンの処理により2個体の四倍体を得た.<br> 1.'楠'実生の長さ3~10 cmに達した新梢先端部に対する0.005%APMの14日間の浸漬処理および0.2%コルヒチンの14日間の浸漬処理,2%の4日間の浸漬処理により四倍体をそれぞれ1個体得た.<br> 2.'富房'成木の発芽・伸長期の新梢先端部に対する0.005%APMの14日間の浸漬処理により四倍体を1個体得た.<br> 3.幼根の長さが3~4 cmの'楠'発根種子の根に対する0.005%APMの14日間の浸漬処理により,四倍体を1個体得た.<br> 4.2週間APM無添加MS培地で馴化培養した23~24年生'楠'成木の新梢茎頂組織を0.005%APMを含むMS培地に移植し,7日間処理することにより四倍体を1個体得た.<br>

This experiment was conducted to artificially induce loquat tetraploids by applying amiprofos-methyl (APM) and colchicine for chromosome doubling agents to diploid loquat shoot apex.<br> Germinated shoot tips from the seeds of the diploid loquat ‘Kusunoki’ were immersed in aqueous solutions of APM or colchicine, and one tetraploid was produced by either immersing 14 shoot tips in a 0.2% colchicine solution for 14 days, 48 shoot tips in a 2% colchicine solution for 4 days, or 30 shoot tips in a 0.005% APM solution for 14 days. Young intact on-tree shoot tips of an 11-year-old diploid loquat ‘Tomifusa’ were immersed in a 0.005% APM solution for 14 days, one tetraploid was obtained from 50 shoots treated. ‘Kusunoki’ seeds on growing young roots were planted on a culture plate containing 0.005% APM solution for 14 days, and one tetraploid was obtained from 50 seeds treated. Apical meristems removed from young intact on-tree shoots of ‘Kusunoki’ cultured on Murashige and Skoog (MS) agar medium for 14 days were treated with APM by transplanting on MS agar medium containing 0.005% APM for 7 days. By this treatment, one tetraploid was obtained from 21 meristems treated.<br>

Journal

  • Horticultural Research (Japan)

    Horticultural Research (Japan) 3(4), 339-344, 2004-12-15

    THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE

References:  17

Cited by:  2

Codes

  • NII Article ID (NAID)
    110001815869
  • NII NACSIS-CAT ID (NCID)
    AA11608561
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    13472658
  • NDL Article ID
    7184177
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産)
  • NDL Call No.
    Z74-C757
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE  NDL-Digital  JASI 
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